Cause and Importance of the Age-dependent Sarcopenia With a Blood Based Bioenergetics & Cytokine Profiling Approach
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 150
- 试验地点
- 1
- 主要终点
- Differences in mitochondrial respiratory capacity of peripheral blood mononuclear cells (PBMCs) using Oxygraph-2k respirometry
研究概览
简要总结
The goal of this cross-sectional study is to evaluate the difference in cytokine profiling approach and mitochondrial function between geriatric sarcopenic patients (group 1) and geriatric non-sarcopenic patients (group 2) and healthy (group 3) geriatric participants.
详细描述
Study participants (group 1&2) are assessed during the hospital stay and age-matched controls (group 3) will be recruited through Flyers.
The investigators aim to isolate and cryopreserve peripheral blood mononuclear cells (PBMCs) from whole blood to perform functional assays on mitochondrial respiratory capacity and ROS. Furthermore, the investigators will investigate the association between biomarkers and frailty, quality of life, functional performance, fatigue and risk of depression.
The investigators hypothesize that this profiling approach will allow the evaluation of the progression of age-dependent sarcopenia and uncover novel therapeutic targets.
研究设计
- 研究类型
- Observational
- 观察模型
- Cohort
- 时间视角
- Cross Sectional
入排标准
- 年龄范围
- 60 Years 至 85 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Age between 60 and 85 years
- •Informed written consent
- •Normal cognitive status according to Mini-Mental State Examination (MMSE ≥ 24 points)
- •Life expectancy of > 3 months according to treating doctor
- •without implanted Defibrillation
- •without acute Sepsis
- •without neurodegenerative diseases, especially amyotrophic lateral sclerosis (ALS) and Huntington
排除标准
- •Age < 60 years or > 85 years
- •Lack of informed written consent
- •Low cognitive status according to Mini-Mental State Examination (MMSE < 24 points)
- •Life expectancy of < 3 months according to treating doctor
- •Implanted Defibrillation
- •Acute Sepsis
- •neurodegenerative diseases, especially amyotrophic lateral sclerosis (ALS) and Huntington
结局指标
主要结局
Differences in mitochondrial respiratory capacity of peripheral blood mononuclear cells (PBMCs) using Oxygraph-2k respirometry
时间窗: Baseline
次要结局
- Frailty Status according to Fried et.al. criteria(Baseline)
- Quality of life (validated questionnaire)(Baseline)
- Functional limitations (single validated questionnaire)(Baseline)
研究者
Kristina Norman
PD Dr.
Charite University, Berlin, Germany