Platelet Rich Fibrin/Biphasic Calcium Phosphate Impairs Osteoclasts Differentiation and Promotes Apoptosis by the Intrinsic Mitochondrial Pathway in Chronic Periodontitis
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 发起方
- 入组人数
- 50
- 主要终点
- probing pocket depth
研究概览
简要总结
The present study is an effort to investigate the hypothesis that Platelet Rich Fibrin/Biphasic Calcium Phosphate may play a role in inhibiting osteoclasts differentiation and in bone loss.
详细描述
Study design
Fifty-five individuals from a suburban population were recruited. Out of them, 5 individuals refused to participate in the study. Finally, 50 participants were selected and divided into two groups, control group [Healthy individuals]: 25 Males/Females, aged 25 to 35 years and test group [Chronic periodontitis patients]: 25 Males/Females aged 30 to 45 years.
Demographic variables and periodontal parameters were measured in both the groups.The investigators explored the effect of Platelet rich fibrin (PRF)/Biphasic calcium phosphate (BCP) on differentiation and survival of osteoclasts obtained from peripheral blood of Chronic Periodontitis patients.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Parallel
- 主要目的
- Other
- 盲法
- None
入排标准
- 年龄范围
- 25 Years 至 35 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •(Control group)
- •Subjects with age group between 30 to 45 years.
- •Non-smokers.
- •No previous history of periodontal treatment.
- •Inclusion Criteria: (Test group)
- •Subjects with age group between 30 to 45 years.
- •Presence of atleast 20 teeth
- •More than 30% of the sites with clinical attachment loss.
- •Non-smokers.
- •No previous history of periodontal treatment.
排除标准
- •( both the groups)
- •Individuals with systemic disease
- •Subjects under antibiotics or anti-inflammatory drugs within the previous 3 months.
- •Pregnant and lactating women.
- •Allergic to antibiotics.
结局指标
主要结局
probing pocket depth
时间窗: base line
probing pocket dept was measured using Williams periodontal probe at base line
次要结局
- Protein expression on osteoclast cells was estimated with BCP, PRF alone and BCP/PRF combination.(24 hours from baseline)
- The activity of caspase-3 in OCs.(24 hours from base line)
研究者
Dr.Jaideep Mahendra
Professor, Director post graduate studies
Meenakshi Ammal Dental College and Hospital