Prehabilitative Exercise Prior to Immobilization
- Conditions
- Disuse AtrophySarcopeniaSkeletal Muscle Atrophy
- Interventions
- Other: Prehabilitative exercise
- Registration Number
- NCT06205784
- Lead Sponsor
- Bispebjerg Hospital
- Brief Summary
This is a 10-week human study involving 24 younger (20-35 y) and 24 older (65-85 y) healthy individuals. All participants will undergo unilateral immobilization of a knee for 7-10 days, followed by 4 weeks of heavy resistance exercise training (HReT). Half of the participants (12 younger and 12 older) will also undergo 4 weeks HReT prior to the immobilization. Prehabilitative exercise may confer protective effects on subsequent immobilization, and the various underlying mechanisms involved
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- RECRUITING
- Sex
- All
- Target Recruitment
- 48
- Age: Between 20-35 years old or between 65-85 years old.
- BMI: Between 18.5 and 35 kg/m2.
- Healthy and well, with normal dietary habits (not vegan).
- More than one muscle biopsy previously taken from both vastus lateralis muscles.
- Smoking.
- Pregnancy.
- Past or present substance or alcohol abuse.
- Medical/surgical conditions that are assessed to affect protein synthesis or the participant's involvement in the trial.
- Knee pain that could affect the completion of the training intervention.
- High intake of supplements that increase the risk of bleeding during muscle biopsy (such as fish oil and ginger).
- Participation in regular strength training within the past year.
- Regular participation in structured sports.
- Current use of blood-thinning medication.
- Previous participation in trials involving oral intake of heavy water.
- Metal in the body preventing MRI scanning.
- For the older group, not having gone through menopause, and for the younger group, not having a regular menstrual cycle.
Study & Design
- Study Type
- INTERVENTIONAL
- Study Design
- PARALLEL
- Arm && Interventions
Group Intervention Description Prehab Prehabilitative exercise -
- Primary Outcome Measures
Name Time Method Quadriceps cross-sectional area From pre (week 0) to post immobilization (week 5) Quadriceps cross-sectional area measured by MRI
- Secondary Outcome Measures
Name Time Method Lean-body mass From pre (week 0) to post immobilization (week 5) and post retraining (week 10) Whole-body and thigh lean-body mass assessed using DEXA-scans
Muscle strength From pre (week 0) to post immobilization (week 5) and post retraining (week 10) Muscle strength during knee extension assessed using isokinetic and isometric dynamometers
Denervated muscle fibers From pre (week 0) to post immobilization (week 5) and post retraining (week 10) Assessment denervated fibers using histochemical stainings
Muscle fiber size From pre (week 0) to post immobilization (week 5) and post retraining (week 10) Muscle fiber size assessed by histochemical stainings
Muscle gene expression From pre (week 0) to post immobilization (week 5) and post retraining (week 10) Muscle gene expression assessed by RNA sequencing
Tendon ultrastructure From pre immobilization (week 4) to post immobilization (week 5) Tendon tissue analysed by molecular analyses
Muscle protein synthesis From pre immobilization (week 4) to post immobilization (week 5) Muscle protein synthesis assessed using deuterium (heavy water)
Tendon structure From pre (week 0) to post immobilization (week 5) Tendon structure measured by MRI
Balance From pre (week 0) to post immobilization (week 5) and post retraining (week 10) One-legged balanced assessed using a force platform
Force variability From pre (week 0) to post immobilization (week 5) and post retraining (week 10) Force variability during knee extension assessed using submaximal isometric contractions
Brain structure From pre (week 0) to post immobilization (week 5) and post retraining (week 10) Brain structure measured by MRI
Brain function From pre (week 0) to post immobilization (week 5) and post retraining (week 10) Brain function measured by MRI
Trial Locations
- Locations (1)
Institute of Sports Medicine Copenhagen
🇩🇰Copenhagen, Capital Region, Denmark