The human body facing and defending from chemical skin allergens reacting by alternative mechanisms: understanding from the molecule to the tissue * an explorative pilot study

Withdrawn

Conditions: contactallergy / contactdermatitis
10014982

Registration Number: NL-OMON45820

Lead Sponsor: niversitair Medisch Centrum Groningen

Brief Summary: Not available

Detailed Description: Not available

Recruitment & Eligibility

Status: Withdrawn

Sex: Not specified

Target Recruitment: 10

Inclusion Criteria

Case group:
- A proven contact allergy (by previous patch testing at the department of dermatology in the UMCG) to either/both FM I and/or FM II.
- Caucasian female
- Age between 20 and 50 years
- Legal competence;Control group:
- Caucasian female
- Age between 20 and 50 years
- No clinical history of dermatitis reaction to fragranced products
- Legal competence

Exclusion Criteria

- Skin-anomalies on the forearm such as active eczema
- Current atopic dermatitis or psoriasis
- The use of immunosuppressive medication during the study or in the four weeks
before inclusion (oral corticosteroids, cyclosporine, methotrexate, azathioprine and
biologicals)
- Legally incompetent
- Difficulty understanding spoken and written information in Dutch.

Study & Design

Study Type: Observational invasive

Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
<p>Keratinocytes from the biopsies of allergic individuals and controls will be<br /><br>isolated and propagated in vitro. They will be cocultured with the dendritic<br /><br>cell surrogate THP-1 cells and exposed to purified terpene hydroperoxides. As<br /><br>main study parameters/endpoints will be the release of inflammatory mediators<br /><br>and expression of activation markers in and on both types. </p><br>

Secondary Outcome Measures