Effect of the Menstrual Cycle on Levels of Growth Factors From Platelet-rich Fibrin and the Size Outcomes of Membrane
试验速览
- 阶段
- 不适用
- 状态
- 尚未招募
- 入组人数
- 36
- 主要终点
- Changes in PDGF in the obtained PRF during the menstrual cycle
研究概览
简要总结
The goal of this clinical trial is to test effect of menstrual cycle (MC) on growth factors of platelet rich fibrin (PRF) in health condition. The main questions it aims to answer are:
question 1: Does MC have an effect on growth factors in PRF? question 2: In which menstrual phase are growth factors higher? question 3:Does the change in the number of blood cells in the menstrual cycle have an effect on PRF? Participants will describe the main tasks participants will be asked to do.
详细描述
Abstract Platelet-rich fibrin (PRF) is frequently used in periodontal regenerative treatments. Thanks to the growth factors in its content, it affects the healing positively. Studies have shown that the size and content of PRF are affected by variables such as age, gender, centrifugation speed and inflammation.
It is known that during the menstrual cycle (MS) phases in women, changes occur in cell numbers and growth factors in the blood. The aim of this study is to determine the size of the PRF and the epidermal growth factor (EGF), insulin-like growth factor-1 (IGF-1), platelet-derived growth factor-BB (PDGF-BB), transforming growth factor-β1 (TGF-β1) and vascular endothelial growth factor (VEGF).
PRF membrane will be formed from the blood of each individual in the study, taken on days 1-2 (M), 8-10 (PF), 12-14 (OD) and days 22-24 before menstruation (PmD). The size of the membranes will be measured. On the same days, individuals will have a complete blood count. The growth factors levels of each PRF membrane obtained at the 1st, 24th and 72nd hours will be determined.
At the end of the study, the growth factors contents of the PRF membranes obtained in different phases of MS will be compared. In addition, the effect of the results of the complete blood count of the individuals on the PRF membrane will be evaluated.
研究设计
- 研究类型
- Observational
- 观察模型
- Cohort
- 时间视角
- Prospective
入排标准
- 年龄范围
- 20 Years 至 35 Years（Adult）
- 性别
- Female
- 接受健康志愿者
- 否
入选标准
- •Having a regular MS in the last three periods and a menstrual period lasting 5-7 days,
- •Not using contraceptive drugs and/or drugs that affect sex hormones,
- •With less than 10% bleeding on probing within 10 seconds after probing, that is, gingival ally healthy,
- •Have not received any periodontal or orthodontic treatment until at least 6 months before the start of the study,
- •Have not used antibiotics in the last 6 months for any reason
- •Systemically healthy individuals without prosthetic restoration in their mouths will be included.
排除标准
- •Those who have any systemic disease and drug use related to it
- •Pregnant or breastfeeding
- •have recently used aspirin or have a disease related to the clotting process
- •Refusing to participate in the study
结局指标
主要结局
Changes in PDGF in the obtained PRF during the menstrual cycle
时间窗: 1 month
PRF membrane will be formed from the blood of each individual in the study, taken on days 1-2 (M), 8-10 (PF), 12-14 (OD) and days 22-24 before menstruation (PmD). PDGF levels of each PRF membrane obtained at the 1st, 24th and 72nd hours will be determined. At the end of the study, PDGF contents of the PRF membranes obtained in different phases of MS will be compared.
Changes in VEGF in the obtained PRF during the menstrual cycle
时间窗: 1 month
PRF membrane will be formed from the blood of each individual in the study, taken on days 1-2 (M), 8-10 (PF), 12-14 (OD) and days 22-24 before menstruation (PmD). VEGF levels of each PRF membrane obtained at the 1st, 24th and 72nd hours will be determined. At the end of the study, VEGF contents of the PRF membranes obtained in different phases of MS will be compared.
Changes in EGF in the obtained PRF during the menstrual cycle
时间窗: 1 month
PRF membrane will be formed from the blood of each individual in the study, taken on days 1-2 (M), 8-10 (PF), 12-14 (OD) and days 22-24 before menstruation (PmD). EGF levels of each PRF membrane obtained at the 1st, 24th and 72nd hours will be determined. At the end of the study, EGF contents of the PRF membranes obtained in different phases of MS will be compared.
Changes in IGF-1 in the obtained PRF during the menstrual cycle
时间窗: 1 month
PRF membrane will be formed from the blood of each individual in the study, taken on days 1-2 (M), 8-10 (PF), 12-14 (OD) and days 22-24 before menstruation (PmD). IGF-1 levels of each PRF membrane obtained at the 1st, 24th and 72nd hours will be determined.At the end of the study, IGF-1 contents of the PRF membranes obtained in different phases of MS will be compared.
Changes in TGF-beta in the obtained PRF during the menstrual cycle
时间窗: 1 month
PRF membrane will be formed from the blood of each individual in the study, taken on days 1-2 (M), 8-10 (PF), 12-14 (OD) and days 22-24 before menstruation (PmD). TGF-beta levels of each PRF membrane obtained at the 1st, 24th and 72nd hours will be determined. At the end of the study, TGF-beta contents of the PRF membranes obtained in different phases of MS will be compared.
次要结局
- Change in the proportion of red blood cells and its effect on PRF.(1 month)
研究者
Zeynep Tastan Eroglu
assistant professor
Necmettin Erbakan University