Metabolism of Branched-chain Amino Acids in Monocyte and Macrophage During Sepsis
试验速览
- 阶段
- 不适用
- 入组人数
- 90
- 主要终点
- Intracellular levels of branched-chain amino acids in monocytes
研究概览
简要总结
The current project was designed to examine the metabolic level of branched-chain amino acids in plasm and monocyte/macrophage, and its role in immune dysfunction during sepsis.
详细描述
In this study, the investigators will collect the peripheral blood of 1,3,7,14 day after diagnose of sepsis. Then, the concentration of branched-chain amino acids(BCAAs) in monocytes and plasma, and the expression of BCAAs metabolic enzymes in monocytes will be determined. Additionally, the inflammatory cytokines, e.g., Interleukin-1β (IL-1β), Tumor necrosis factor-α (TNF-α), Interleukin-10 (IL-10）, the chemokines macrophage inflammatory proteins 1α and 1β (MIP-1α and 1β) in the peripheral blood and the phagocytic function of monocytes will also be detected. The current project was designed to examine the metabolic level of branched-chain amino acids in plasm and monocyte/macrophage, and its role in immune dysfunction during sepsis.
研究设计
- 研究类型
- Observational
- 观察模型
- Case Control
- 时间视角
- Prospective
入排标准
- 年龄范围
- 18 Years 至 70 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •patients diagnosed with sepsis, with or without type 2 diabetes
- •healthy volunteers
排除标准
- •patients with immunodeficient diseases
- •patients who accept glucocorticoid or immunosuppressant
- •patients with an age younger than 18 years
- •patients who cannot provide the informed consent
结局指标
主要结局
Intracellular levels of branched-chain amino acids in monocytes
时间窗: up to 14 day after diagnose of sepsis
BCAAs Assay Kit will be used to measure the intracellular levels of BCAAs in monocytes.
次要结局
- The phagocytic index of monocytes.(1,3,5,7,10,14 day after diagnose of sepsis.)
- The plasma level of chemokine MIP-1β.(1,3,5,7,10,14 day after diagnose of sepsis.)
- The expression of branched-chain α-ketoacid dehydrogenase in monocytes(1,3,5,7,10,14 day after diagnose of sepsis)
- The plasma level of chemokine MIP-1α.(1,3,5,7,10,14 day after diagnose of sepsis.)
- The plasma level of TNF-α(1,3,5,7,10,14 day after diagnose of sepsis.)
- Plasma levels of branched-chain amino acids(1,3,5,7,10,14 day after diagnose of sepsis)
- The plasma level of IL-1β(1,3,5,7,10,14 day after diagnose of sepsis.)
- The plasma level of IL-10(1,3,5,7,10,14 day after diagnose of sepsis.)
- The expression of branched-chain amino acid aminotransferase in monocytes(1,3,5,7,10,14 day after diagnose of sepsis)
- The expression of PP2Cm in monocytes(1,3,5,7,10,14 day after diagnose of sepsis)
研究者
Chunling Jiang
Clinical Professor
West China Hospital