Effect of Different Sperm Processing Methods in ICSI Outcome.
- Conditions
- Sperm DNA Fragmentation
- Registration Number
- NCT03668106
- Lead Sponsor
- Benha University
- Brief Summary
A total 180 semen samples from couples diagnosed with unexplained infertility were SDF index tested , then total 120 semen samples of patients' husbands with abnormal SDF index were randomly divided and then processed by swim up, sperm gradient centrifugation and Zeta methods. SDF and ICSI outcomes are monitored after semen processing.
- Detailed Description
A total 180 semen samples from couples diagnosed with unexplained infertility were SDF index tested , then total 120 semen samples of patients' husbands with abnormal SDF index were randomly divided and then processed by swim up (G1/ n=40), sperm gradient centrifugation (G2/ n=40) and Zeta (G3/ n=40) methods. SDF test was assessed by Halosperm kit, whereas sperm morphology was assessed by spermac stain according to strict criteria. Fertilization, division, blastulation, implantation and pregnancy rates will be tabulated and statistically tested.
Recruitment & Eligibility
- Status
- TERMINATED
- Sex
- All
- Target Recruitment
- 20
- unexplained infertile couples for 2 years
- age from 20 years old to 37 years old
- endometriosis cases
- uterine factors
Study & Design
- Study Type
- INTERVENTIONAL
- Study Design
- PARALLEL
- Primary Outcome Measures
Name Time Method Sperm DNA fragmentation 30 minutes measure sperm integrity by halosperm assay
- Secondary Outcome Measures
Name Time Method Blastulation rate day 5 no of embryos blastulated / total no. of embryos
pregnancy rate 15 days no of pregnant cases\\ all no. of cases
Oocyte fertilization rate day 1 no. of oocytes fertilized /total no. of oocytes
Trial Locations
- Locations (1)
Ahmed Saad
🇪🇬Banha, Qalubiya, Egypt
Ahmed Saad🇪🇬Banha, Qalubiya, Egypt