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Adipose Tissue Function and Response to Exercise Training in Women With and Without Polycystic Ovary Syndrome

Not Applicable
Completed
Conditions
Polycystic Ovary Syndrome
Interventions
Behavioral: 4x4 minutes high intensity interval training
Behavioral: 10x1 minute high intensity interval training
Registration Number
NCT02943291
Lead Sponsor
Norwegian University of Science and Technology
Brief Summary

The primary aim of this trial is to investigate adipose tissue function in women with and without polycystic ovary syndrome (PCOS). PCOS is a common endocrine disorder in young women. The pathogenesis behind PCOS is complex and only partly understood, and deeper mechanistic insight is needed. Insulin resistance is a central feature of PCOS, and recent studies have suggested that this is linked to aberrant adipose tissue function. Exercise training has been found to improve the symptoms in PCOS, but we need more knowledge about why. While processes involved in skeletal muscle oxidative remodeling are well described, it is to a large extent unknown whether the oxidative capacity of human adipose tissue is modified by endurance training. The women included in this study will be matched (for body mass index, body weight, and age) to participants in another study. This will enable the investigators to do a comparison between cases (women with PCOS) and controls (women without PCOS) at baseline, and to assess the responses to exercise training in adipose tissue.

Detailed Description

Not available

Recruitment & Eligibility

Status
COMPLETED
Sex
Female
Target Recruitment
15
Inclusion Criteria
  • eumenorrheic
  • matching PCOC group in IMPROV-IT study (NCT02419482)
  • Living nearby St Olavs Hospital, Trondheim, Norway
Exclusion Criteria
  • Signs of hyperandrogenism
  • Regular high intensity endurance (two or more times per week of vigorous exercise).
  • Concurrent treatments (insulin sensitizers or drugs known to affect gonadotropin or ovulation, with a wash out period of 1 months prior to inclusion).
  • On-going pregnancy
  • Hormonal contraception
  • Breastfeeding within 24 weeks

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
4x4 minutes interval training4x4 minutes high intensity interval training4x4 minutes high intensity interval training with 4 minute intervals
10x1 minute interval training10x1 minute high intensity interval training10x1 minute high intensity interval training with 1 minute intervals
Primary Outcome Measures
NameTimeMethod
mitochondrial oxidative phosphorylation capacity16 weeks

assessed with high resolution respirometry

production of reactive oxygen species16 weeks

assessed with high resolution respirometry

Secondary Outcome Measures
NameTimeMethod
blood glucose16 weeks
Adipokine secretion16 weeks
lipid droplet size in adipocytes16 weeks
Whole-body peak oxygen uptake16 weeks
Adipokine gene expression16 weeks
blood pressure16 weeks
Intima media thickness16 weeks

measured by ultrasound

HDL cholesterol in blood16 weeks
Body composition16 weeks

assessed by impedance scale (InBody)

Glycosylated Hemoglobin (HbA1c) in blood16 weeks
Whole-body fat oxydation rate16 weeks
Insulin sensitivity16 weeks

2h oral glucose tolerance test (OGGT)

Total protein abundance in adipose tissue16 weeks

quantified using standard Western Blot method

"blood markers of cardiometabolic health"16 weeks
blood insulin16 weeks
endothelial function16 weeks

measured by flow-mediated dilatation of the brachial artery

Total cholesterol in blood16 weeks
LDL cholesterol in blood16 weeks

Trial Locations

Locations (2)

Centre of Exercise and Nutrition, Mary MacKillop Institute for Health Research, AUC

🇦🇺

Melbourne, Australia

Department of circulation and medical imaging , NTNU

🇳🇴

Trondheim, Norway

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