Adipose Tissue Cellular Response to Exercise in Healthy Humans
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 40
- 试验地点
- 2
- 主要终点
- Quantifying the change in markers of macrophage abundance in adipose tissue
研究概览
简要总结
The purpose of this study is to measure the effects of a single session of exercise on changes in the cellular composition of the body fat of humans.
详细描述
Fat tissue is far more than just a place where humans store body fat. Fat tissue contains many different types of cells, such as immune cells, endothelial cells, and fat precursor cells. Differences or changes in the number of immune cells, endothelial cells, and fat precursor cells in fat tissue can have a great impact on metabolic health. The abundance of all immune cells, endothelial cells, and fat precursor cell types are known to change when a person loses or gains weight. For example, the abundance of inflammatory cells is often found to decrease markedly with weight loss - and this change has been linked to the profound improvements in metabolic health that occur with weight loss. In contrast, the effects of exercise on immune cells, endothelial cells, and fat precursor cells in fat tissue is not known. Determining the effects of exercise on changes in the cellular composition within fat tissue will provide important information for optimizing lifestyle interventions aimed at improving metabolic health.
研究设计
- 研究类型
- Interventional
- 分配方式
- Na
- 干预模型
- Single Group
- 主要目的
- Basic Science
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 40 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Age: 18-40
- •Body Mass Index: 20-27 kg/m2
- •Regular exercise (less than or equal to 3 days/week of aerobic exercise; 30-60 minutes/session at moderate and vigorous intensities)
- •Women must have regularly occurring menses and must be premenopausal
排除标准
- •Blood pressure greater than 140/90 mm Hg
- •Pregnant or lactating
- •Evidence/history of cardiovascular or metabolic disease
- •Medications known to affect lipid or glucose metabolism, or inflammation
结局指标
主要结局
Quantifying the change in markers of macrophage abundance in adipose tissue
时间窗: 1 day
The stromovascular fraction will be isolated from subcutaneous lipo-aspirate samples by collagenase digestion and a series of filtration/wash steps (routinely performed by our lab). This isolation protocol has been optimized to yield the minimum number of stromal cells required to identify and recover the following cell populations by fluorescence-activated cell sorting (FACS), listed as cell surface stain = cell type: CD64+/CD11c+ = macrophages FACS will be performed at the University of Michigan Flow Cytometry Core, and sorted cells will be recovered for subsequent gene expression analysis using the StepOnePlus real time PCR system (Life Technologies) with fluorescent probe-based assays.
次要结局
- Quantifying the change in markers of endothelial cell and pre-adipocytes in adipose tissue(1 day)
研究者
Jeffrey F Horowitz
Associate Professor, Movement Science, School of Kinesiology
University of Michigan