Molecular Analysis of Liver Cancer

Brief Summary

Detailed Description

OBJECTIVES: * To characterize, at a molecular level, archived samples of tissue from young patients with fibrolamellar carcinoma and hepatocellular carcinoma in non-cirrhotic livers matched for age and sex. * To perform genomic analysis on these tissue samples using array comparative genomic hybridization. * To perform targeted gene mutation analysis on these samples by PCR. * To perform proteomic profiling on fixed tissues in these samples by various proteomic methods, including IHC and mass spectrometry. * To look for association between molecular aberrations and clinicopathologic features in these samples. OUTLINE: Archived tissue samples are collected from the pathology department at Vanderbilt University Medical Center and from the Mayo Clinic in Rochester, Minnesota. Tissue samples are analyzed by genomic analysis using array comparative genomic hybridization, target gene mutation analysis by PCR, and proteomic profiling on fixed tissues using various proteomic methods, including IHC and mass spectrometry. Samples are also examined for association between molecular aberrations and clinicopathologic features found in each disease. Clinical patient data (i.e., age, sex, race, date of diagnosis, risk factors, histology, surgical staging, follow-ups, date of death, and adjuvant therapy) are also collected.

Primary Outcomes

Secondary Outcomes

• Association between fibrolamellar carcinoma and hepatocellular carcinoma in terms of molecular aberrations and clinicopathologic features(After molecular analysis of tissue and after collection of clinicopathologic data)