Platelet Enzymatically Oxidized Phospholipids (eoxPL) Characterisation in a Healthy Cohort on and Off Aspirin

Not Applicable

Completed

• Conditions: Platelet Dysfunction Due to Aspirin
• Interventions: Drug: Aspirin

• Registration Number: NCT05604118
• Lead Sponsor: Cardiff University

Brief Summary

Healthy volunteers (n = 28) were recruited from the workplace (14 male, 14 female; age range 20-50 years). Ethical approval, which included informed consent, was from Cardiff University, Schools of Medicine and Dentistry Research Ethics Committee (SMREC16/02). Following a 2-week period free from non-steroidal anti-inflammatory drugs (NSAIDs), peripheral blood was obtained and platelets isolated. Participants were commenced on aspirin 75 mg once daily for seven days and then provided a repeat blood sample. Following a 2-month period, participants were invited to repeat samples pre- and post-aspirin, and again a third time 2 months later.

Detailed Description

Not available

Study Timeline

• Study Start: 2016-08-01
• Primary Completion: 2018-08-01
• Study Completion: 2022-06-01
• Status Verified: 2022-10
• Study First Submitted: 2022-10-20
• Study First Submitted QC: 2022-10-27
• Last Update Submitted: 2022-10-27
• Study First Posted: 2022-11-03
• Last Update Posted: 2022-11-03

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 28

Inclusion Criteria

• two-week washout period from any non-steroidal anti-inflammatory drugs (NSAIDs) use.
• Ability to take aspirin 75 mg once daily for seven days and sampled thereafter, before stopping the aspirin.
• Willingness to repeat this process after two months and four months of the initial sampling date.

Exclusion Criteria

* Recent NSAID use

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: CROSSOVER

Arm && Interventions

Group	Intervention	Description
Aspirin supplemented	Aspirin	-

Primary Outcome Measures

Name	Time	Method
Platelet EoxPL differences in amounts (ng) as measured by liquid chromatography with tandem mass spectrometry	4 months	Samples on and off aspirin will be quantified for the 47 most common eoxPL species (as described by Slatter et al, Cell Metabolism 23, 930-944, May 10, 2016) using established liquid chromatography with tandem mass spectrometry (LC-MS/MS). Differences in amounts on and off aspirin will be quantified and tested for statistical significance.

Trial Locations

Locations (1)

Sir Geraint Evans Cardiovascular Research Building; 🇬🇧 Cardiff, United Kingdom