An in vitro investigation to assess the osteoanabolic effects of doxycycline in a proteosomal gel form.
Not Applicable
- Registration Number
- CTRI/2024/07/071118
- Lead Sponsor
- Dr Vaishnavi Langde self
- Brief Summary
Not available
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- ot Yet Recruiting
- Sex
- Not specified
- Target Recruitment
- 0
Inclusion Criteria
Healthy dental pulp mesenchymal stem cells procured from healthy non carious extracted tooth.
Exclusion Criteria
NI
Study & Design
- Study Type
- Observational
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method Invitro evaluation of the biological behavior of doxycycline proniosomal gel for their use as bone regenerative material.Timepoint: 1,2and 6 months
- Secondary Outcome Measures
Name Time Method 1.To evaluate the cytotoxic effect of doxycycline proniosomal gel on pulpal dental stem cells by MTT assay. <br/ ><br>2.To study the osteogenic differentiation potential of doxycycline proniosomal gel in mesenchymal stem cells by alizarin red staining. <br/ ><br>3.To investigate the osteogenic gene expression such as alkaline phosphatase and osteonectin for osteogenic differentiation. <br/ ><br>Timepoint: 1,2 and 6 months