Regulation of muscle oxidative phenotype by hypoxia in Chronic Obstructive Pulmonary Disease and Chronic Heart Failure
- Conditions
- heart failurelung emphysema100192801002830210038716
- Registration Number
- NL-OMON33801
- Lead Sponsor
- niversiteit Maastricht
- Brief Summary
Not available
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- Completed
- Sex
- Not specified
- Target Recruitment
- 90
COPD patients: inclusion criterion for COPD patients is COPD according to GOLD criteria.
Heart failure patients: inclusion criterion for CHF patients is diagnosis heart failure with an ejection fraction < 40% determined by echocardiography
COPD patients: exclusion criteria for COPD patients are malignancy, cardiac failure, distal arteriopathy, recent surgery, severe endocrine, hepatic or renal disorders, oxygen therapy and recent participation in a revalidation program (previous 6 months).
CHF patients: exclusion criteria for CHF patients are unstable disease, unstable angina pectoris, correctable cause of heart failure or valvular heart disease, restrictive or hypertrophic cardiomyopathy, malignancy, pulmonary disease (including primary pulmonary hypertension and COPD), distal arteriopathy, recent surgery, severe endocrine, hepatic or renal disorders and recent participation in a revalidation program (previous 6 months).
Patients with artificial valves, known left ventricular thrombi and mitral stenosis will also be excluded.
Study & Design
- Study Type
- Observational invasive
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method <p>Main outcome parameters are the skeletal muscle expression levels of HIF-1a,<br /><br>PGC-1 and PPARs in the different groups. </p><br>
- Secondary Outcome Measures
Name Time Method <p>Also, markers of hypoxia such as vascular endothelial growth factor, carbonic<br /><br>anhydrase-9 and heme oxygenase-1 will be measured. Metabolic enzyme activities<br /><br>and muscle fiber type proportions will be determined to assess oxidative<br /><br>phenotype. Outcome parameters regarding adipose tissue are adipocyte size and<br /><br>cell surface, expression levels of markers for hypoxia like HIF-1a and GLUT-1,<br /><br>and expression levels of inflammatory mediators like TNF-a, IL-6, leptin and<br /><br>adiponectin.</p><br>