Effects of PA-1 Transcriptional Regulation on Monocyte Function

Terminated

• Conditions: Atherosclerosis

• Registration Number: NCT00512369
• Lead Sponsor: Vanderbilt University

Brief Summary

Researching genetic differences in people with no prior medical conditions for better understanding of cardiac diseases through genetic research.

Detailed Description

The discovery and subsequent application of small interfering RNA (siRNA) methods to achieve individual gene silencing in mammalian cells is a robust method that is well-described and validated in mammalian cell culture systems. We will apply this technique to achieve post-transcriptional "silencing" of PAI-1 in monocytes obtained from otherwise healthy volunteers, and study the subsequent loss of this gene's function on the migratory capacity of these cells in the proposed in vitro experimental system. This concept has not yet been demonstrated in the literature, and if validated, would be a novel and fundamental description of the role of PAI-1 in human monocyte biology as related to the development of atherosclerosis.

Study Timeline

• Study Start: 2006-03
• Study First Submitted: 2007-08-03
• Study First Submitted QC: 2007-08-06
• Study First Posted: 2007-08-07
• Status Verified: 2011-07
• Study Completion: 2011-07
• Last Update Submitted: 2011-07-14
• Last Update Posted: 2011-07-18

Recruitment & Eligibility

• Status: TERMINATED
• Sex: All
• Target Recruitment: 125

Inclusion Criteria

• Ages 18 -50
• No current or past medical problems

Exclusion Criteria

• Patients taking prescription drugs (hormonal birth control or herbal supplements may be taken.

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Trial Locations

Locations (1)

Vanderbilt University Medical Center; 🇺🇸 Nashville, Tennessee, United States