Impact of Exercise-induced Energetic Stress During an 8-hour Fast on Metabolic Regulation in Resting Human Skeletal Muscle
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 10
- 试验地点
- 2
- 主要终点
- Peroxisome proliferator activated receptor gamma co-activator 1-alpha (PGC-1α) messenger ribonucleic acid (mRNA) expression
研究概览
简要总结
This study examines the impact of increasing energetic stress during an 8-hour fast on metabolic pathways in human skeletal muscle. Specifically, participants will undergo two 8-hour fasting sessions in a randomized crossover fashion, with the addition of low-intensity arm exercise in one of the sessions. Changes in metabolic pathways will be examined in resting (leg) muscle before, during, and after the fast.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Crossover
- 主要目的
- Basic Science
- 盲法
- Double (Investigator, Outcomes Assessor)
盲法说明
Allocation concealment, assessor blinding
入排标准
- 年龄范围
- 18 Years 至 30 Years（Adult）
- 性别
- Male
- 接受健康志愿者
- 是
入选标准
- •recreationally active (no more than 3 hours of weekly physical activity)
- •not currently involved in a systematic training program
- •body mass index < 30 kg/m2
排除标准
- •presence of cardiometabolic disease
- •taking regular oral medication
- •current smoker
研究组 & 干预措施
FAST - FAST+EX
FAST alone then FAST+EX
干预措施: FAST+EX (Other)
FAST - FAST+EX
FAST alone then FAST+EX
干预措施: FAST (Other)
FAST+EX - FAST
FAST+EX then FAST alone
干预措施: FAST+EX (Other)
FAST+EX - FAST
FAST+EX then FAST alone
干预措施: FAST (Other)
结局指标
主要结局
Peroxisome proliferator activated receptor gamma co-activator 1-alpha (PGC-1α) messenger ribonucleic acid (mRNA) expression
时间窗: 15 min before fast; after 4 and 8 hours of fasting
PGC-1α gene expression (indirect indicator of PGC-1α activity)
次要结局
- phosphorylated protein kinase A (PKA) substrates(15 min before fast; after 4 and 8 hours of fasting)
- Catecholamine concentrations(15 min before fast; after exercise; after 4 and 8 hours of fasting)
- total and phosphorylated AMP activated protein kinase (AMPK)(15 min before fast; after 4 and 8 hours of fasting)
- total and phosphorylated SIRT-1(15 min before fast; after 4 and 8 hours of fasting)
- total and phosphorylated Akt/Protein Kinase B(15 min before fast; after 4 and 8 hours of fasting)
- total and phosphorylated p38 mitogen activated protein kinase (MAPK)(15 min before fast; after 4 and 8 hours of fasting)
- General control non-repressible 5 (GCN5) mRNA expression(15 min before fast; after 4 and 8 hours of fasting)
- PGC-1α protein content(15 min before fast; after 4 and 8 hours of fasting)
- Intramuscular triglyceride (IMTG) content(15 min before fast; after 4 and 8 hours of fasting)
- Glucose and insulin concentrations(15 min before fast; after 4 and 8 hours of fasting)
- total and phosphorylated mammalian target of rapamycin (mTOR)(15 min before fast; after 4 and 8 hours of fasting)
- total and phosphorylated acetyl coenzyme A carboxylase (ACC)(15 min before fast; after 4 and 8 hours of fasting)
- Pyruvate dehydrogenase kinase isoenzyme 4 (PDK4) mRNA expression(15 min before fast; after 4 and 8 hours of fasting)
- p53 mRNA expression(15 min before fast; after 4 and 8 hours of fasting)
- Leucine rich pentatricopeptide repeat containing protein (LRP130) mRNA expression(15 min before fast; after 4 and 8 hours of fasting)
- acetylated p53(15 min before fast; after 4 and 8 hours of fasting)
- Sirtuin 1 (SIRT1) mRNA expression(15 min before fast; after 4 and 8 hours of fasting)
- Intramuscular glycogen content(15 min before fast; after 4 and 8 hours of fasting)
- Sirtuin 3 (SIRT3) mRNA expression(15 min before fast; after 4 and 8 hours of fasting)
- Cytochrome C oxidase subunit 4 (COX-IV) protein content(15 min before fast; after 4 and 8 hours of fasting)
研究者
Brendon Gurd
Associate Professor
Queen's University