Impact of Exercise-induced Energetic Stress During an 8-hour Fast on Metabolic Regulation in Resting Human Skeletal Muscle

Queen's University1 site in 1 country10 target enrollmentJanuary 14, 2019

ConditionsSkeletal Muscle Energetics

Overview

Phase: Not Applicable
Intervention: Not specified
Conditions: Skeletal Muscle Energetics
Sponsor: Queen's University
Enrollment: 10
Locations: 1
Primary Endpoint: Peroxisome proliferator activated receptor gamma co-activator 1-alpha (PGC-1α) messenger ribonucleic acid (mRNA) expression
Status: Completed
Last Updated: 6 years ago

Overview Investigators Eligibility Criteria Outcomes Sites Similar Trials

Overview

Brief Summary

This study examines the impact of increasing energetic stress during an 8-hour fast on metabolic pathways in human skeletal muscle. Specifically, participants will undergo two 8-hour fasting sessions in a randomized crossover fashion, with the addition of low-intensity arm exercise in one of the sessions. Changes in metabolic pathways will be examined in resting (leg) muscle before, during, and after the fast.

Registry

clinicaltrials.gov

Start Date

January 14, 2019

End Date

April 30, 2019

Last Updated

6 years ago

Study Type

Interventional

Study Design

Crossover

Sex

Male

Investigators

Sponsor

Queen's University

Responsible Party

Principal Investigator

Brendon Gurd

Associate Professor

Queen's University

Eligibility Criteria

Inclusion Criteria

•recreationally active (no more than 3 hours of weekly physical activity)
•not currently involved in a systematic training program
•body mass index \< 30 kg/m2

Exclusion Criteria

•presence of cardiometabolic disease
•taking regular oral medication
•current smoker

Outcomes

Primary Outcomes

Peroxisome proliferator activated receptor gamma co-activator 1-alpha (PGC-1α) messenger ribonucleic acid (mRNA) expression

Time Frame: 15 min before fast; after 4 and 8 hours of fasting

PGC-1α gene expression (indirect indicator of PGC-1α activity)

Secondary Outcomes

phosphorylated protein kinase A (PKA) substrates(15 min before fast; after 4 and 8 hours of fasting)
Catecholamine concentrations(15 min before fast; after exercise; after 4 and 8 hours of fasting)
total and phosphorylated AMP activated protein kinase (AMPK)(15 min before fast; after 4 and 8 hours of fasting)
total and phosphorylated SIRT-1(15 min before fast; after 4 and 8 hours of fasting)
total and phosphorylated Akt/Protein Kinase B(15 min before fast; after 4 and 8 hours of fasting)
total and phosphorylated p38 mitogen activated protein kinase (MAPK)(15 min before fast; after 4 and 8 hours of fasting)
General control non-repressible 5 (GCN5) mRNA expression(15 min before fast; after 4 and 8 hours of fasting)
PGC-1α protein content(15 min before fast; after 4 and 8 hours of fasting)
Intramuscular triglyceride (IMTG) content(15 min before fast; after 4 and 8 hours of fasting)
Glucose and insulin concentrations(15 min before fast; after 4 and 8 hours of fasting)
total and phosphorylated mammalian target of rapamycin (mTOR)(15 min before fast; after 4 and 8 hours of fasting)
total and phosphorylated acetyl coenzyme A carboxylase (ACC)(15 min before fast; after 4 and 8 hours of fasting)
Pyruvate dehydrogenase kinase isoenzyme 4 (PDK4) mRNA expression(15 min before fast; after 4 and 8 hours of fasting)
p53 mRNA expression(15 min before fast; after 4 and 8 hours of fasting)
Leucine rich pentatricopeptide repeat containing protein (LRP130) mRNA expression(15 min before fast; after 4 and 8 hours of fasting)
acetylated p53(15 min before fast; after 4 and 8 hours of fasting)
Sirtuin 1 (SIRT1) mRNA expression(15 min before fast; after 4 and 8 hours of fasting)
Intramuscular glycogen content(15 min before fast; after 4 and 8 hours of fasting)
Sirtuin 3 (SIRT3) mRNA expression(15 min before fast; after 4 and 8 hours of fasting)
Cytochrome C oxidase subunit 4 (COX-IV) protein content(15 min before fast; after 4 and 8 hours of fasting)