The effect of cold acclimation on human brown adipose tissue - a comparative study in obesity and type 2 daibetes
Recruiting
- Conditions
- Brown adipose tissue, Cold acclimation, Skeletal muscle, Non-shivering, Thermogenesis, Diabetes, Obesity
- Registration Number
- NL-OMON22725
- Lead Sponsor
- Maastricht University Medical Center + (MUMC+)
- Brief Summary
/A
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- Recruiting
- Sex
- Not specified
- Target Recruitment
- 36
Inclusion Criteria
General
1. Male
Exclusion Criteria
1. Participate in physical activity more than 2x/week
2. Participation in earlier research that included a PET/CT-scan
Study & Design
- Study Type
- Interventional
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method 1. Standard uptake values (SUVs) of FDG of active brown adipose tissue with the PET/CT-scans, one time prior and one time post the cold acclimation period (2x 2hour);<br /><br>2. Energy expenditure with indirect calorimetry, one time prior and one time post the cold acclimation period (3x 3hour);<br /><br>3. Skeletal muscle mitochondrial uncoupling with the muscle biopsy, one time before and one time post the cold acclimation period (2x 30minutes). BRITE cell recruitment with the fat biopsy, one time prior and one time post the cold acclimation period (2x 30minutes). <br /><br>4. Glucose infusion rate (ml/h) by hyperinsulinemic euglycemic clamp, one time prior and one time post the cold acclimation period (2x 5hour)<br />
- Secondary Outcome Measures
Name Time Method 1. Body temperatures (core and skin temperatures) with iButtons and a telemetric pill, one time prior and one time post the cold acclimation period;<br /><br>2. Skin perfusion with laser Doppler flowmetry, one time prior and one time post the cold acclimation period;<br /><br>3. Blood parameters, among others insulin, glucose, free fatty acids and thyroid hormones, one time prior and post the cold acclimation period;<br /><br>4. Body composition with a DXA-scan, one time prior and post the cold acclimation period. <br />