GCF Levels of Chemokines in Periodontitis

Completed

• Conditions: Periodontal Diseases
• Interventions: Diagnostic Test: Routine periodontal examination

• Registration Number: NCT06291454
• Lead Sponsor: T.C. ORDU ÜNİVERSİTESİ

Brief Summary

This study aimed to determine CCL19/MIP3beta(β) and CCL20/MIP3alpha(α) in the gingival crevicular fluid (GCF) of the patients with different periodontitis phenotypes.

A total of 72 individuals, 24 with Stage III Grade B periodontitis (Group 1), 24 with Stage III Grade C periodontitis (Group 2), and 24 periodontally healthy (control group) (Group 3), were included in the study. GCF and clinical periodontal parameters were obtained at baseline. MIP3β and MIP3α levels in GCF were determined by ELISA.

Detailed Description

Not available

Study Timeline

• Study Start: 2023-01-01
• Primary Completion: 2023-12-01
• Study Completion: 2023-12-01
• Study First Submitted: 2024-02-22
• Study First Submitted QC: 2024-02-29
• Status Verified: 2024-03
• Study First Posted: 2024-03-04
• Last Update Submitted: 2024-03-06
• Last Update Posted: 2024-03-08

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 72

Inclusion Criteria

• Have at least 20 natural teeth, excluding third molars.
• Periodontitis patients had at least two non-adjacent sites per quadrant with probing depth (PD) ≥ 5 mm and clinical attachment level (CAL) ≥ 5 mm with gingival inflammation, and alveolar bone loss affecting >30% of the teeth, as detected on clinical and radiographical examinations.
• Periodontally healthy control group had no sign of gingival inflammation, no PD > 3mm and no evidence of attachment or bone loss

Exclusion Criteria

• History of systemic disease.
• Regular use of any drugs which can effect the immune system or inflammatory response in the 6 months preceding the start of the study.
• Periodontal treatment during last 6 months that could affect periodontal status.
• Smoking.
• History of radiotherapy or chemotherapy.
• Current pregnancy, lactation or menopause.

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Arm && Interventions

Group	Intervention	Description
Stage III Grade B periodontitis group (Group 1)	Routine periodontal examination	Stage III Grade B periodontitis patients and systemically healthy
Stage III Grade C periodontitis group (Group 2)	Routine periodontal examination	Stage III Grade C periodontitis patients and systemically healthy
Control group (Group 3)	Routine periodontal examination	Periodontally healthy (control group) and systemically healthy patients

Primary Outcome Measures

Name	Time	Method
CCL20/MIP3alpha(α) levels	Baseline	CCL20/MIP3alpha(α) levels in the gingival crevicular fluid (GCF)
CCL19/MIP3beta(β) levels	Baseline	CCL19/MIP3beta(β) levels in the gingival crevicular fluid (GCF) of the all groups

Secondary Outcome Measures

Name	Time	Method
CCL19/MIP3beta(β) levels and clinical parameters	Baseline	Correlation between CCL19/MIP3beta(β) levels in the gingival crevicular fluid (GCF) of the all groups and all clinical parameters
CCL20/MIP3alpha(α) levels and clinical parameters	Baseline	Correlation between CCL20/MIP3alpha(α) levels in the gingival crevicular fluid (GCF) of the all groups and all clinical parameters

Trial Locations

Locations (1)

Ordu University, Faculty of Dentistry; 🇹🇷 Ordu, Turkey