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Histamine H1/H2 Receptors and Training Adaptations

Not Applicable
Completed
Conditions
Exercise Training
Physical Activity
Interventions
Other: Lactose
Other: High-intensity interval training (HIIT)
Registration Number
NCT04450134
Lead Sponsor
University Ghent
Brief Summary

Exercise training is beneficial for both health and performance. Histamine has been shown to be involved in the acute exercise response. The current study addresses the role of histamine H1/H2 receptor signaling in the chronic training-induced adaptations. Results from this study will yield more insights into the molecular mechanisms of adaptations to exercise training.

Detailed Description

Not available

Recruitment & Eligibility

Status
COMPLETED
Sex
Male
Target Recruitment
19
Inclusion Criteria
  • Sedentary or low levels of physical activity
  • Caucasian
Exclusion Criteria
  • Chronic diseases
  • Medication use
  • Smoking
  • Excessive alcohol consumption
  • Seasonal allergies

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
PlaceboHigh-intensity interval training (HIIT)6 weeks high-intensity interval training + placebo intake
PlaceboLactose6 weeks high-intensity interval training + placebo intake
BlockadeHigh-intensity interval training (HIIT)6 weeks high-intensity interval training + histamine H1/H2 receptor blockade
BlockadeFexofenadine Hydrochloride6 weeks high-intensity interval training + histamine H1/H2 receptor blockade
BlockadeFamotidine6 weeks high-intensity interval training + histamine H1/H2 receptor blockade
Primary Outcome Measures
NameTimeMethod
Change in cardiorespiratory fitnessBefore, after 3 weeks and after 6 weeks of exercise training

Change in maximal oxygen uptake during incremental cycling test on cycle ergometer during the 6 week training period

Change in peak aerobic power outputBefore, after 3 weeks and after 6 weeks of exercise training

Change in peak power output during incremental cycling test on cycle ergometer during the 6 week training period

Change in whole-body insulin sensitivityBefore and after 6 weeks of exercise training

Change from baseline in Matsuda index for whole-body insulin sensitivity derived from Oral Glucose Tolerance Test after the 6 week training period

Change in microvascular functionBefore and after 6 weeks of exercise training

Change from baseline in microvascular function (Single Passive Leg Movement technique) after the 6 week training period

Secondary Outcome Measures
NameTimeMethod
Change in substrate oxidation during submaximal cyclingBefore and after 6 weeks of exercise training

Change from baseline in substrate oxidation during submaximal cycling test (estimated via gas exchange data) after the 6 week training period

Change in blood lactate accumulation during submaximal cyclingBefore and after 6 weeks of exercise training

Change from baseline in capillary lactate concentration at end of submaximal cycling test after the 6 week training period

Change in fasted serum glucose concentrationsBefore and after 6 weeks of exercise training

Change from baseline in fasted blood concentrations of glucose after the 6 week training period

Change in body weightBefore and after 6 weeks of exercise training

Change from baseline in total body weight after the 6 week training period

Change in skeletal muscle protein contentBefore and after 6 weeks of exercise training

Change from baseline in Western Blot assessment of markers of relevance for skeletal muscle function after the 6 week training period

Change in skeletal muscle capillarizationBefore and after 6 weeks of exercise training

Change from baseline in skeletal muscle capillarization (immunohistochemistry) after the 6 week training period

Change in power output at Gas Exchange Threshold (GET)Before, after 3 weeks and after 6 weeks of exercise training

Change from baseline in GET during incremental cycling test after the 6 week training period

Change in skeletal muscle enzyme activityBefore and after 6 weeks of exercise training

Change from baseline in enzyme activity assessment of markers of relevance for skeletal muscle function after the 6 week training period

Change in power output at Respiratory Compensation Point (RCP)Before, after 3 weeks and after 6 weeks of exercise training

Change from baseline in RCP during incremental cycling test after the 6 week training period

Change in time to exhaustion performance testBefore, after 3 weeks and after 6 weeks of exercise training

Change in time to exhaustion test (performed after incremental cycling test) during the 6 week training period

Change in heart rate during submaximal cyclingBefore and after 6 weeks of exercise training

Change from baseline in heart rate during submaximal cycling after the 6 week training period

Change in cycling efficiency during submaximal cyclingBefore and after 6 weeks of exercise training

Change from baseline in cycling efficiency (estimated via gas exchange data) after the 6 week training period

Change in fasted serum insulin concentrationsBefore and after 6 weeks of exercise training

Change from baseline in fasted blood concentrations of insulin after the 6 week training period

Change in fasted serum cholesterol concentrationsBefore and after 6 weeks of exercise training

Change from baseline in fasted blood concentrations of cholesterol after the 6 week training period

Change in fasted serum triglyceride concentrationsBefore and after 6 weeks of exercise training

Change from baseline in fasted blood concentrations of triglyceride after the 6 week training period

Change in resting heart rateBefore and after 6 weeks of exercise training

Change from baseline in resting heart rate after the 6 week training period

Change in resting blood pressureBefore and after 6 weeks of exercise training

Change from baseline in resting mean arterial blood pressure after the 6 week training period

Trial Locations

Locations (1)

Department of Movement and Sports Sciences, Ghent University

🇧🇪

Gent, Oost-Vlaanderen, Belgium

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