Physiological Effect of Ataulfo Mango (Manguifera Indica) Beverage in Exercise

Not Applicable

Completed

• Conditions: Exercise-induced Oxidative Stress
• Interventions: Dietary Supplement: Mango pulp beverage; Dietary Supplement: Mango pulp and peel beverage; Dietary Supplement: Control beverage

• Registration Number: NCT03182543
• Lead Sponsor: Universidad Autonoma de Ciudad Juarez

Brief Summary

The aim of this study is to assess the antioxidant capacity of two mango base beverages (AM1, AM2), against exercise-induced oxidative stress.

Detailed Description

Introduction. The post-consumption physiological effect of mango cv. "Ataulfo" pulp (MP) and peel (MC) in humans before exercise has been poorly evaluated.

Objective. To evaluate the physiological response, to consumption of two mango base beverages, AM1 (200g MP/ 600mL water) and AM2 (160g MP; 40g MC / 600mL water), before and after a submaximal exercise test.

Methods. The antioxidant profile and physicochemical characteristics of AM1 and AM2 were evaluated. Several cardiorespiratory, anthropometric and body composition parameters of 19 university students, prior to the consumption of AM1, AM2 and water, were evaluated in three non-consecutive sessions (self-control cases). The glycemic, lactic and antioxidant (FRAP), uric acid (AU), reduced glutathione (GSH) and lipid oxidation (TBARS) and protein carbonyls (PC) responses were evaluated in plasma before and after an incremental cycle ergometer exercise test.

Study Timeline

• Study Start: 2017-01-01
• Primary Completion: 2017-02-28
• Study Completion: 2017-04-30
• Study First Submitted: 2017-05-29
• Status Verified: 2017-06
• Study First Submitted QC: 2017-06-07
• Last Update Submitted: 2017-06-07
• Study First Posted: 2017-06-09
• Last Update Posted: 2017-06-09

Recruitment & Eligibility

• Status: COMPLETED
• Sex: Male
• Target Recruitment: 19

Inclusion Criteria

• healthy male
• university student
• no alcohol, cigar or drugs consumption

Exclusion Criteria

• no antioxidant supplement or ergogenic consumption
• health issues with performing physical activity

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: CROSSOVER

Arm && Interventions

Group	Intervention	Description
AM1	Mango pulp beverage	Mango pulp beverage
AM2	Mango pulp and peel beverage	Mango pulp and peel beverage
Control	Control beverage	Control beverage

Primary Outcome Measures

Name	Time	Method
Antioxidant capacity change	0 min (before beverage intake), 9-15 (immediately after exercise test)	Assessed by reduced glutathione concentration change. Blood samples were drawn from the antecubital vein into EDTA test tubes at rest, before beverage intake, and immediately following the exercise test. Samples were then centrifuged at 5000 rpm for 10 min at 4°C to obtain plasma, which was withdrawn and separated into eppendorf vials, maintained at -80°C until further biochemical analysis.

Secondary Outcome Measures

Name	Time	Method
Oxidative stress change	0 min (before beverage intake), 9-15 (immediately after exercise test)	Assessed by protein carbonyls concentration change. Blood samples were drawn from the antecubital vein into EDTA test tubes at rest, before beverage intake, and immediately following the exercise test. Samples were then centrifuged at 5000 rpm for 10 min at 4°C to obtain plasma, which was withdrawn and separated into eppendorf vials, maintained at -80°C until further biochemical analysis.
Lactic acid concentration change	0 min (before performing exercise test), 3 min, 6 min, 9 min, 12 min, 15 min (during exercise test)	Assessed by lactic acid concentration change. Capillary blood samples were taken from the fingertip, using a lancet and analyzed (YSI modelo 1500, Yellow Springs, USA)
Postprandial glycemic change	0 min (before beverage intake), 30 min, 60 min, 90 min, 120 min (after beverage intake)	Assessed by blood glucose concentration change. Capillary blood samples were taken from the fingertip, using a lancet, and analyzed (ReliOn Confirm/micro Test Strips ARKRAY, USA)