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HS-IT101 Injection for Advanced NSCLC

Not Applicable
Not yet recruiting
Conditions
Advanced Non-Small Cell Lung Cancer
Interventions
Registration Number
NCT07105176
Lead Sponsor
Qingdao Sino-Cell Biomedicine Co., Ltd.
Brief Summary

An Open-Label, Single-Arm Phase Ib Clinical Trial Evaluating the Safety, Tolerability, and Preliminary Efficacy of HS-IT101 Injection in Subjects with Advanced NSCLC.

Detailed Description

A Single-Arm, Open-Label, Interventional Study Evaluating Adoptive Cell Therapy (ACT) with Autologous Tumor-Infiltrating Lymphocytes (HS-IT101) Following Lymphodepleting Conditioning with Fludarabine and Cyclophosphamide, Followed by IL-2 in Patients with Advanced NSCLC.

Recruitment & Eligibility

Status
NOT_YET_RECRUITING
Sex
All
Target Recruitment
12
Inclusion Criteria
  • Age: 18-70 years (inclusive).

Diagnosis:

Histologically/cytologically confirmed advanced, recurrent, or metastatic non-small cell lung cancer (NSCLC) .

Tumor Sampling:

≥1 lesion untreated with radiotherapy/local therapy within 28 days for TIL preparation (tissue weight ≥0.050 g).

Target Lesion:

≥1 measurable lesion per RECIST v1.1, untreated with radiotherapy/local therapy (unless treatment occurred >28 days before sampling with documented progression).

Performance Status: ECOG score ≤1. Survival: Life expectancy ≥3 months.

Organ Function:

Hematology: ANC ≥1.5×10⁹/L, PLT ≥90×10⁹/L, HGB ≥90 g/L (no transfusion/erythropoietin within 14 days).

Liver: ALT/AST ≤2.5×ULN (≤5×ULN if liver metastases); TBil ≤1.5×ULN (≤3×ULN for Gilbert syndrome).

Kidney: Serum Cr ≤1.5×ULN or Ccr ≥60 mL/min (Cockcroft-Gault formula). Coagulation: APTT ≤1.5×ULN; INR/PT ≤1.5×ULN.

Cardiac Function:

LVEF ≥50% by echocardiography; QTcF ≤470 ms (Fridericia formula: QTcF = QT/RR⁰·³³).

Baseline SpO₂ >91% (room air). Note: If QTcF is abnormal initially, repeat twice at ≥5-minute intervals and use mean value for eligibility.

Toxicity Recovery: All treatment-related adverse events resolved to CTCAE v5.0 ≤Grade 1 (except alopecia/non-risk toxicities per investigator) before tumor sampling.

Contraception: Effective non-pharmacological contraception from informed consent until 1 year post-TIL infusion.

Compliance: Capable of understanding the trial, voluntarily signing informed consent, and adhering to protocol visits/procedures.

Exclusion Criteria
  • Severe Hypersensitivity: History of severe hypersensitivity to drugs used in the study (including but not limited to cyclophosphamide, fludarabine, IL-2, gentamicin, amphotericin B, or components of TIL infusion).

Uncontrolled Comorbidities:

Poorly controlled hypertension (resting SBP ≥160 mmHg or DBP ≥100 mmHg despite medication).

Congestive heart failure (NYHA Class III/IV).

Cardiovascular Events (within 6 months):

Deep vein thrombosis, pulmonary embolism, myocardial infarction, severe/unstable arrhythmia, angina, PCI, ACS, CABG, stroke, TIA, or cerebral embolism.

Active Autoimmune Disease:

Requires systemic therapy during the study period (Exceptions: Eczema, vitiligo, psoriasis, alopecia, or Graves' disease stable without systemic therapy for 2 years; hypothyroidism on hormone replacement; type 1 diabetes on insulin).

Transplantation History: Solid organ or hematopoietic stem cell transplantation.

Immunosuppressive Therapy:

Use of immunosuppressants (e.g., steroids) within 4 weeks before tumor sampling (Allowed: Physiologic glucocorticoid doses ≤12 mg/m²/day hydrocortisone equivalent; topical/nasal steroids).

Recent Anticancer Therapy:

Systemic anticancer treatment within 4 weeks before preconditioning (including investigational drugs; washout <5 half-lives if <4 weeks).

Planned participation in other interventional trials.

Active Infections:

HIV/syphilis antibody-positive; active HBV/HCV (Allowed: HBsAg/HBeAg+ if HBV DNA below LLN; HCV Ab+ if HCV RNA below LLN).

Active systemic infection or tuberculosis requiring treatment. Recent Surgery/Trauma: Major surgery or significant trauma within 4 weeks before screening; elective surgery planned during the study.

Poor Wound Healing: Surgery-related complications or delayed healing increasing risks of TIL therapy (per investigator judgment).

Other Malignancies: Additional primary malignancy within 5 years (Exceptions: Curatively treated basal/squamous cell carcinoma or carcinoma in situ).

Severe Respiratory Disease: History of severe ILD, COPD, pulmonary insufficiency, or symptomatic bronchospasm.

Gastrointestinal Complications: Surgical-required GI bleeding, bowel ischemia, or perforation.

CNS Involvement:

Leptomeningeal metastasis; uncontrolled/untreated CNS metastases (Exceptions: Asymptomatic lesions <1 cm, stable for ≥4 weeks without steroids/anticonvulsants).

Prior Cell Therapy: Previous treatment with similar cellular products. Pregnancy/Lactation: Pregnant or breastfeeding women.

Other Exclusions:

Psychiatric disorders, alcoholism, drug abuse, or other conditions deemed unsuitable by the investigator.

Study & Design

Study Type
INTERVENTIONAL
Study Design
SINGLE_GROUP
Arm && Interventions
GroupInterventionDescription
HS-IT101 monotherapyCyclophosphamideAdoptive transfer of 5x10\^9-6x10\^10 autologous TIL to patients i.v. in 30-60 minutes.
HS-IT101 monotherapyFludarabineAdoptive transfer of 5x10\^9-6x10\^10 autologous TIL to patients i.v. in 30-60 minutes.
HS-IT101 monotherapyIL-2 (interleukin 2)Adoptive transfer of 5x10\^9-6x10\^10 autologous TIL to patients i.v. in 30-60 minutes.
HS-IT101 monotherapyCyclophosphamideTIL Injection administered by intravenous infusion over 30-60 minutes.
HS-IT101 monotherapyFludarabineTIL Injection administered by intravenous infusion over 30-60 minutes.
HS-IT101 monotherapyHS-IT101 monotherapyTIL Injection administered by intravenous infusion over 30-60 minutes.
HS-IT101 monotherapyIL-2 (interleukin 2)TIL Injection administered by intravenous infusion over 30-60 minutes.
Primary Outcome Measures
NameTimeMethod
Adverse Events (AE)12 months

To characterize the safety profile of HS-IT101 in patients with advanced solid tumor as assessed by incidence of adverse events

Serious Adverse Events (SAE)12 months

To characterize the safety profile of HS-IT101 in patients with advanced solid tumor as assessed by incidence of serious adverse events

Objective Response Rate (ORR)Up to 36 months

To evaluate the efficacy of HS-IT101 in patients with advanced solid tumor, based on the objective response rate (ORR) as assessed by the Independent Review Committee (IRC) per RECIST v1.1

Time-to-response (TTR)Up to 36 months

To evaluate the efficacy of HS-IT101 in patients with advanced solid tumor by assessing the time-to-response (TTR) as assessed by the Investigator per RECIST v1.1

Duration of Response (DOR)Up to 36 months

To evaluate the efficacy of HS-IT101 in patients with advanced solid tumor by assessing the duration of response (DOR) as assessed by the Investigator per RECIST v1.1

Disease Control Rate (DCR)Up to 36 months

To evaluate the efficacy of HS-IT101 in patients with advanced solid tumor, based on the disease control rate (DCR) as assessed by the Independent Review Committee (IRC) per RECIST v1.1

Secondary Outcome Measures
NameTimeMethod
Progression-Free-Survival (PFS)Up to 36 months

To evaluate progression-free-survival (PFS) in patients with advanced solid tumor

Overall Survival (OS)Up to 36 months

To evaluate overall survival (OS) in patients with advanced solid tumor

Pharmacokinetic (PK) detection parameters for HS-IT101Up to 6 months

T-cell receptor (TCR) clonality

Trial Locations

Locations (1)

Guangdong Provincial People's Hospital

🇨🇳

Guangzhou, Guangdong, China

Guangdong Provincial People's Hospital
🇨🇳Guangzhou, Guangdong, China
YiLong Wu
Contact
86-020-83525173
gdghospital_ec@gdph.org.cn

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