Effect of Amino Acids and Sugar Alcohols on Satiation Peptides and Activation of Specific Brain Regions

Not Applicable

Completed

• Conditions: Endocrine System Diseases
• Interventions: Dietary Supplement: 1.56 g L-Leucine; Dietary Supplement: 50 g Xylitol; Dietary Supplement: 1.56 g L-Tryptophan; Dietary Supplement: 75 g Erythritol; Dietary Supplement: 75 g Glucose; Dietary Supplement: Tap water; Dietary Supplement: Mixed liquid meal (Ensure Plus®; 17% protein, 29% fat and 54% carbohydrate); Device: Nasogastric Tube

• Registration Number: NCT02823249
• Lead Sponsor: University Hospital, Basel, Switzerland

Brief Summary

With this study the investigators investigate the effects of amino acids (tryptophan, leucin) and sugar alcohols (xylitol, erythritol) on satiety mechanisms and brain activation.

Detailed Description

Not available

Study Timeline

• Study Start: 2014-05
• Primary Completion: 2015-04
• Study Completion: 2015-04
• Study First Submitted: 2016-06-27
• Study First Submitted QC: 2016-07-05
• Study First Posted: 2016-07-06
• Status Verified: 2017-02
• Last Update Submitted: 2017-02-26
• Last Update Posted: 2017-02-28

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 20

Inclusion Criteria

• Healthy normal weight subjects with a body-mass index of 19.0-24.9
• Healthy obese subjects with a body-mass index of > 30
• Normal eating habits (no diets; no dietary changes; no special dietary habits such as vegetarian/vegan)
• Age 18-40 years
• Stable body weight for at least three months

Exclusion Criteria

• Smoking
• Substance abuse
• Regular intake of medications (except for oral contraceptives)
• Medical or psychiatric illness
• History of gastrointestinal disorders
• Food allergies
• Pregnancy, breast feeding
• Body piercings that cannot be removed
• History of claustrophobia
• Left-hander

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: CROSSOVER

Arm && Interventions

Group	Intervention	Description
1.56 g L-Tryptophan	Tap water	1.56 g L-Tryptophan in 300 mL tap water given via nasogastric tube + after 60 min: 500ml of a mixed liquid meal (Ensure Plus®; 17% protein, 29% fat and 54% carbohydrate) given via nasogastric tube
1.56 g L-Leucine	1.56 g L-Leucine	1.56 g L-Leucine in 300 mL tap water given via nasogastric tube + after 60 min: 500ml of a mixed liquid meal (Ensure Plus®; 17% protein, 29% fat and 54% carbohydrate) given via nasogastric tube
75 g Erythritol	Tap water	75 g Erythritol in 300 mL tap water given via nasogastric tube
50 g Xylitol	50 g Xylitol	50 g Xylitol in 300 mL tap water given via nasogastric tube
75 g Glucose and mixed liquid meal	Tap water	75 g Glucose in 300 mL tap water given via nasogastric tube + after 60 min: 500ml of a mixed liquid meal (Ensure Plus®; 17% protein, 29% fat and 54% carbohydrate) given via nasogastric tube
1.56 g L-Tryptophan	1.56 g L-Tryptophan	1.56 g L-Tryptophan in 300 mL tap water given via nasogastric tube + after 60 min: 500ml of a mixed liquid meal (Ensure Plus®; 17% protein, 29% fat and 54% carbohydrate) given via nasogastric tube
1.56 g L-Tryptophan	Mixed liquid meal (Ensure Plus®; 17% protein, 29% fat and 54% carbohydrate)	1.56 g L-Tryptophan in 300 mL tap water given via nasogastric tube + after 60 min: 500ml of a mixed liquid meal (Ensure Plus®; 17% protein, 29% fat and 54% carbohydrate) given via nasogastric tube
1.56 g L-Tryptophan	Nasogastric Tube	1.56 g L-Tryptophan in 300 mL tap water given via nasogastric tube + after 60 min: 500ml of a mixed liquid meal (Ensure Plus®; 17% protein, 29% fat and 54% carbohydrate) given via nasogastric tube
75 g Erythritol	75 g Erythritol	75 g Erythritol in 300 mL tap water given via nasogastric tube
75 g Glucose and mixed liquid meal	75 g Glucose	75 g Glucose in 300 mL tap water given via nasogastric tube + after 60 min: 500ml of a mixed liquid meal (Ensure Plus®; 17% protein, 29% fat and 54% carbohydrate) given via nasogastric tube
1.56 g L-Leucine	Mixed liquid meal (Ensure Plus®; 17% protein, 29% fat and 54% carbohydrate)	1.56 g L-Leucine in 300 mL tap water given via nasogastric tube + after 60 min: 500ml of a mixed liquid meal (Ensure Plus®; 17% protein, 29% fat and 54% carbohydrate) given via nasogastric tube
50 g Xylitol	Tap water	50 g Xylitol in 300 mL tap water given via nasogastric tube
50 g Xylitol	Nasogastric Tube	50 g Xylitol in 300 mL tap water given via nasogastric tube
75 g Glucose and mixed liquid meal	Mixed liquid meal (Ensure Plus®; 17% protein, 29% fat and 54% carbohydrate)	75 g Glucose in 300 mL tap water given via nasogastric tube + after 60 min: 500ml of a mixed liquid meal (Ensure Plus®; 17% protein, 29% fat and 54% carbohydrate) given via nasogastric tube
Tap water and mixed liquid meal	Tap water	300 mL tap water given via nasogastric tube + after 60 min: 500ml of a mixed liquid meal (Ensure Plus®; 17% protein, 29% fat and 54% carbohydrate) given via nasogastric tube
1.56 g L-Leucine	Tap water	1.56 g L-Leucine in 300 mL tap water given via nasogastric tube + after 60 min: 500ml of a mixed liquid meal (Ensure Plus®; 17% protein, 29% fat and 54% carbohydrate) given via nasogastric tube
1.56 g L-Leucine	Nasogastric Tube	1.56 g L-Leucine in 300 mL tap water given via nasogastric tube + after 60 min: 500ml of a mixed liquid meal (Ensure Plus®; 17% protein, 29% fat and 54% carbohydrate) given via nasogastric tube
75 g Erythritol	Nasogastric Tube	75 g Erythritol in 300 mL tap water given via nasogastric tube
75 g Glucose and mixed liquid meal	Nasogastric Tube	75 g Glucose in 300 mL tap water given via nasogastric tube + after 60 min: 500ml of a mixed liquid meal (Ensure Plus®; 17% protein, 29% fat and 54% carbohydrate) given via nasogastric tube
Tap water and mixed liquid meal	Mixed liquid meal (Ensure Plus®; 17% protein, 29% fat and 54% carbohydrate)	300 mL tap water given via nasogastric tube + after 60 min: 500ml of a mixed liquid meal (Ensure Plus®; 17% protein, 29% fat and 54% carbohydrate) given via nasogastric tube
Tap water and mixed liquid meal	Nasogastric Tube	300 mL tap water given via nasogastric tube + after 60 min: 500ml of a mixed liquid meal (Ensure Plus®; 17% protein, 29% fat and 54% carbohydrate) given via nasogastric tube

Primary Outcome Measures

Name	Time	Method
Effect of sugar alcohols on cerebral blood flow measured by functional brain MRI	changes from baseline to one hour after treatment
Effect of aminoacids on cerebral blood flow measured by functional brain MRI	changes from baseline to one hour after treatment

Secondary Outcome Measures

Name	Time	Method
Measurement of Plasma cholecystokinin concentrations	changes from baseline to three hours after treatment
Measurement of Plasma Glucagon-like Peptide (GLP-1) concentrations	changes from baseline to three hours after treatment
Measurement of Plasma PYY concentrations	changes from baseline to three hours after treatment
Measurement of Plasma ghrelin concentrations	changes from baseline to three hours after treatment
Measurement of Plasma Glucose concentrations	changes from baseline to three hours after treatment
Measurement of Plasma Insulin concentrations	changes from baseline to three hours after treatment

Trial Locations

Locations (1)

University Hospital Basel; 🇨🇭 Basel, Basel-Stadt, Switzerland