Effects of a High-fat Meal on Exercise-mediated Sympatholysis

Not Applicable

Recruiting

• Conditions: Diet, Healthy
• Interventions: Dietary Supplement: Control; Dietary Supplement: Beetroot drink; Dietary Supplement: Placebo

• Registration Number: NCT06186050
• Lead Sponsor: University of Guelph

Brief Summary

The goal of this trial is to examine the effects of a single high-fat meal with or without the co-ingestion of dietary nitrate on exercise-mediated sympatholysis. The main questions it aims to answer are:

1. What are the mechanisms responsible for reduced exercise-mediated sympatholysis following a high-fat meal

2. Can dietary nitrate prevent the declines in exercise-mediated sympatholysis

Participants will be asked to complete three study visits in a randomized order:

1. Low-fat meal

2. High-fat meal

3. High-fat meal plus dietary nitrate

Exercise-mediated sympatholysis will be measured using Doppler ultrasound via a protocol involving rhythmic handgrip exercise and a lower body negative pressure stimulus. Sympatholysis will be assessed before each meal, and at 1, 2, and 3 hours post-prandial.

Detailed Description

Not available

Study Timeline

• Study First Submitted: 2023-12-15
• Study First Submitted QC: 2023-12-15
• Study First Posted: 2023-12-29
• Study Start: 2024-01-08
• Status Verified: 2024-05
• Last Update Submitted: 2024-05-07
• Last Update Posted: 2024-05-09
• Primary Completion: 2024-12-30
• Study Completion: 2024-12-30

Recruitment & Eligibility

• Status: RECRUITING
• Sex: All
• Target Recruitment: 15

Inclusion Criteria

• 18-30 years of age
• Healthy, free of known cardiovascular, metabolic, or musculoskeletal disease

Exclusion Criteria

• Diagnosed cardiovascular or metabolic disease, injury preventing handgrip exercise, prescription of chronic medications other than oral contraceptives.
• Inability to abstain from drugs (except oral contraceptives), caffeine, alcohol, strenuous physical activity, mouthwash, and multivitamins prior to study

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: CROSSOVER

Arm && Interventions

Group	Intervention	Description
Low-fat meal control	Control	Kelloggs cornflakes (110g), greek yogurt (400g), skim milk (500 mL), and orange juice (250 mL). Energy-matched to the high-fat meal
High-fat meal with Beetroot Drink	Beetroot drink	2 sausage egg McMuffins and a hashbrown from McDonalds and 10g of beetroot powder mixed in 250 mL cold tap water drink
High-fat meal with Placebo Drink	Placebo	2 sausage egg McMuffins and a hashbrown from McDonalds and Mio water flavouring in 250 mL cold tap water. Trace amounts of sugar and sodium were mixed to match the beetroot supplement

Primary Outcome Measures

Name	Time	Method
Forearm vascular conductance	Before intervention and 1, 2, 3 hours post-intervention	Duplex ultrasound of brachial artery
Systolic and Diastolic Blood Pressure	Before intervention and 1, 2, 3 hours post-intervention	finger plethysmography
Forearm blood flow	Before intervention and 1, 2, 3 hours post-intervention	Duplex ultrasound of brachial artery

Secondary Outcome Measures

Name	Time	Method
Plasma levels of Norepinephrine	Blood collected before intervention and 1, 2, 3 hours post-intervention. Blood frozen at -80 degrees Celsius and analyzed at later date	ELISA kit
Plasma levels of Nitrate/Nitrite concentration	Blood collected before intervention and 1, 2, 3 hours post-intervention. Blood frozen at -80 degrees Celsius and analyzed at later date	ELISA kit
Blood Glucose concentrations	Blood collected before intervention and 1, 2, 3 hours post-intervention. Blood analyzed immediately after collection at each time point	Blood glucose monitor
Plasma levels of Triglycerides	Blood collected before intervention and 1, 2, 3 hours post-intervention. Blood frozen at -80 degrees Celsius and analyzed at later date	Triglyceride assay kit

Trial Locations

Locations (1)

University of Guelph - Human Cardiovascular Physiology Laboratory; 🇨🇦 Guelph, Ontario, Canada