Effects of a High-fat Meal on Exercise-mediated Sympatholysis

Not Applicable

Recruiting

• Conditions: Diet, Healthy

• Registration Number: NCT06186050
• Lead Sponsor: University of Guelph

Brief Summary

The goal of this trial is to examine the effects of a single high-fat meal with or without the co-ingestion of dietary nitrate on exercise-mediated sympatholysis. The main questions it aims to answer are:

1. What are the mechanisms responsible for reduced exercise-mediated sympatholysis following a high-fat meal

2. Can dietary nitrate prevent the declines in exercise-mediated sympatholysis

Participants will be asked to complete three study visits in a randomized order:

1. Low-fat meal

2. High-fat meal

3. High-fat meal plus dietary nitrate

Exercise-mediated sympatholysis will be measured using Doppler ultrasound via a protocol involving rhythmic handgrip exercise and a lower body negative pressure stimulus. Sympatholysis will be assessed before each meal, and at 1, 2, and 3 hours post-prandial.

Detailed Description

Not available

Study Timeline

• Study First Submitted: 2023-12-15
• Study First Submitted QC: 2023-12-15
• Study First Posted: 2023-12-29
• Study Start: 2024-01-08
• Status Verified: 2024-05
• Last Update Submitted: 2024-05-07
• Last Update Posted: 2024-05-09
• Primary Completion: 2024-12-30
• Study Completion: 2024-12-30

Recruitment & Eligibility

• Status: RECRUITING
• Sex: All
• Target Recruitment: 15

Inclusion Criteria

• 18-30 years of age
• Healthy, free of known cardiovascular, metabolic, or musculoskeletal disease

Exclusion Criteria

• Diagnosed cardiovascular or metabolic disease, injury preventing handgrip exercise, prescription of chronic medications other than oral contraceptives.
• Inability to abstain from drugs (except oral contraceptives), caffeine, alcohol, strenuous physical activity, mouthwash, and multivitamins prior to study

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: CROSSOVER

Primary Outcome Measures

Name	Time	Method
Forearm vascular conductance	Before intervention and 1, 2, 3 hours post-intervention	Duplex ultrasound of brachial artery
Forearm blood flow	Before intervention and 1, 2, 3 hours post-intervention	Duplex ultrasound of brachial artery
Systolic and Diastolic Blood Pressure	Before intervention and 1, 2, 3 hours post-intervention	finger plethysmography

Secondary Outcome Measures

Name	Time	Method
Plasma levels of Norepinephrine	Blood collected before intervention and 1, 2, 3 hours post-intervention. Blood frozen at -80 degrees Celsius and analyzed at later date	ELISA kit
Plasma levels of Nitrate/Nitrite concentration	Blood collected before intervention and 1, 2, 3 hours post-intervention. Blood frozen at -80 degrees Celsius and analyzed at later date	ELISA kit
Blood Glucose concentrations	Blood collected before intervention and 1, 2, 3 hours post-intervention. Blood analyzed immediately after collection at each time point	Blood glucose monitor
Plasma levels of Triglycerides	Blood collected before intervention and 1, 2, 3 hours post-intervention. Blood frozen at -80 degrees Celsius and analyzed at later date	Triglyceride assay kit

Trial Locations

Locations (1)

University of Guelph - Human Cardiovascular Physiology Laboratory; 🇨🇦 Guelph, Ontario, Canada