Changes in Alveolar Macrophage Function During Acute Lung Injury

• Conditions: Acute Lung Injury
• Interventions: Procedure: Bronchoscopy

• Registration Number: NCT02693912
• Lead Sponsor: West China Hospital

Brief Summary

In this study, the investigators aim to observe the change in alveolar macrophage function during acute lung injury.

Detailed Description

Alveolar macrophages are tissue-resident or recruited cells with key functions in recognition of pathogens, initiation of host defensive ang protective inflammation,and in clearance of pathogens from the airways. However, macrophages are also responsible for efficient clearance of apoptotic neutrophils, termed efferocytosis, which is a prerequisite for the timely resolution of inflammation. In this study, the investigators aim to observe the change in alveolar macrophage function during acute lung injury.

Study Timeline

• Status Verified: 2016-02
• Study First Submitted: 2016-02-11
• Study First Submitted QC: 2016-02-26
• Last Update Submitted: 2016-02-26
• Study First Posted: 2016-02-29
• Last Update Posted: 2016-02-29
• Study Start: 2016-09
• Primary Completion: 2017-12
• Study Completion: 2018-08

Recruitment & Eligibility

• Status: UNKNOWN
• Sex: All
• Target Recruitment: 25

Inclusion Criteria

• Patients in ICU with diagnosed ALI under mechanical ventilation

Exclusion Criteria

• Patients with cardiac dysfunction, using steroid drugs or other drugs can that affect efferocytosis

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Arm && Interventions

Group	Intervention	Description
control	Bronchoscopy	Patient under mechanical ventilation without any lung diseases. Patients will underwent bronchoscopy for bronchoalveolar lavage fluid.
acute lung injury	Bronchoscopy	Patients with a diagnosis of acute lung injury (ALI) under mechanical ventilation.Patients will underwent bronchoscopy for bronchoalveolar lavage fluid.

Primary Outcome Measures

Name	Time	Method
alveolar macrophages (AMs) phagocytic index	7 days after admission	AMs will be collected and cultured in vitro. Apoptotic neutrophils will then be cocultured with AMs for 1 hour. To measure phagocytosis of apoptotic neutrophils, AMs were further processed and phagocytosed apoptotic neutrophils were detected by confocal fluorescence microscopy as described below under Cell staining and confocal microscopy. The phagocytic index was calculated according to the following formula: phagocytic index = (total number of engulfed cells/total number of counted macrophages) × (number of macrophages containing engulfed cells/total number of counted macrophages) × 100.

Secondary Outcome Measures

Name	Time	Method
30 days mortality	30 days after admission