Phenotypic Characterization of Alveolar Macrophages 2016 (Caract-Aires-2016)

• Conditions: Lung Diseases

• Registration Number: NCT03250897
• Lead Sponsor: Rennes University Hospital

Brief Summary

The aim of the study is to investigate by flow cytometry the phenotype of alveolar macrophages (AMs) isolated from patients with non-infectious lung diseases.

Detailed Description

Although alveolar macrophages (AMs) represent the main immune cell population in the human lungs, consensus on their phenotypic characterization is still missing.

Current translational protocols use human AMs obtained by invasive methods (broncho-alveolar lavage and lung biopsy) or differentiate macrophages from blood monocytes cultured in the presence of growth factors such as macrophage colony-stimulating factor (M-CSF) or granulocyte-macrophage colony-stimulating factor (GM-CSF). The group has already published the phenotypic and functional comparison of human blood monocyte-derived macrophages differentiated with M-CSF or GM-CSF. Aim of this study is to use a multi-staining flow cytometry (FACS analysis) to better characterize the phenotype of AMs isolated from broncho-alveolar lavage fluids of patients affected by non-infectious lung diseases. The FACS profiles of AMs will be further compared to the phenotypic profile of blood monocyte-derived macrophages differentiated in the presence of GM-CSF or M-CSF. The results of this study will allow to define the most valid in vitro model of AMs in a non-infectious setting.

Study Timeline

• Study Start: 2016-11
• Status Verified: 2017-08
• Study First Submitted: 2017-08-11
• Study First Submitted QC: 2017-08-11
• Last Update Submitted: 2017-08-11
• Study First Posted: 2017-08-16
• Last Update Posted: 2017-08-16
• Primary Completion: 2017-12
• Study Completion: 2018-09

Recruitment & Eligibility

• Status: UNKNOWN
• Sex: All
• Target Recruitment: 18

Inclusion Criteria

• Patients affected by non-infectious lung diseases and for whom a broncho-alveolar lavage is scheduled for their medical care in the pulmonary division
• Patient Information leaflet provided
• Written informed consent of the patient

Exclusion Criteria

• A suspected respiratory infection

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
To investigate by flow cytometry the phenotype of alveolar macrophages (AMs) isolated from broncho-alveolar lavage fluids of patients with non-infectious lung diseases.	1 year	Characterization of membrane proteins by multi-staining flow cytometry (FACS analysis)

Secondary Outcome Measures

Name	Time	Method
To compare the phenotypic profile of AMs from broncho-alveolar lavage fluids of patients with non-infectious lung diseases to that of blood monocyte-derived macrophages differentiated in the presence of GM-CSF or M-CSF and obtained from healthy donors	1 year

Trial Locations

Locations (1)

Pneumology Division; 🇫🇷 Rennes, France