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Study to Collect Samples for MIST Analysis of Zibotentan and Bioavailability of Zibotentan and Dapagliflozin in Heatlhy Participants

Phase 1
Completed
Conditions
Chronic Kidney Disease
Interventions
Drug: Zibotentan/Dapagliflozin - Formulation 1 (Treatment B)
Drug: Zibotentan/Dapagliflozin - Formulation 2 (Treatment C)
Registration Number
NCT04991571
Lead Sponsor
AstraZeneca
Brief Summary

The study will have 2 independent parts:

Part 1 of the study is intended to collect samples for Metabolites in Safety Testing (MIST) analysis after administration of multiple doses of zibotentan.

Part 2 of the study is designed to evaluate the relative bioavailability of zibotentan and dapagliflozin after dosing with two different fixed-dose combination (FDC) formulations and dosing with separate formulations of zibotentan and dapagliflozin.

Detailed Description

Part 1 will be an open-label, non-randomised, single treatment period. A single treatment period during which participants will be resident at the study centre from 2 days before dosing (Day -2) until the morning of Day 6.

Part 2 will be an open-label, randomised, 3-period, 3-treatment, cross-over single dose study. Participants will be randomised to one of 3 treatment sequences and will receive 3 single-dose study interventions. Participants will be resident at the study centre from 2 days before dosing (Day -2) until Day 3 of the last treatment sequence.

Participants who were enrolled in Part 1 may not be enrolled in Part 2.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
27
Inclusion Criteria

For inclusion in the study participants should fulfil the following criteria:

  1. Participants with suitable veins for cannulation or repeated venipuncture.
  2. Females must have a negative pregnancy test at the Screening Visit and within 24 hours prior to dosing, must not be lactating and must be of non- childbearing potential
  3. Male participant must adhere to the contraception methods.
  4. Have a BMI between 18 and 29.9 kg/m^2 inclusive and weigh at least 50 kg and no more than 100 kg inclusive.
  5. Provision of signed and dated, written informed consent prior to any study specific procedures.
Exclusion Criteria

Participants will not enter the study if any of the following exclusion criteria are fulfilled:

  1. History or presence of gastrointestinal, hepatic or renal disease or any important disease or disorder.

  2. Any clinically important illness, medical/surgical procedure or trauma within 4 weeks of the first administration of study intervention.

  3. Any clinically significant abnormalities in clinical chemistry, haematology, or urinalysis.

  4. Any positive result on screening for serum hepatitis B surface antigen, hepatitis C antibody, and Human immunodeficiency virus antibody.

  5. Abnormal vital signs. 6 History of drug abuse or alcohol abuse.

  6. History of severe allergy/hypersensitivity or ongoing clinically important allergy/hypersensitivity.

  7. Participants who are vegans or have medical dietary restrictions. 9. Participants tested positive for COVID-19 at the time of randomisation or have been previously hospitalised with COVID-19 infection.

Study & Design

Study Type
INTERVENTIONAL
Study Design
CROSSOVER
Arm && Interventions
GroupInterventionDescription
Part 2: Treatment Sequence BCAZibotentan (Treatment A)Each participant will receive 3 single-dose treatments of zibotentan and dapagliflozin (Treatment B; Treatment C; Treatment A) in 3 treatment periods, separated by a washout period of at least 7 days between treatment periods.
Part 2: Treatment Sequence CABZibotentan (Treatment A)Each participant will receive 3 single-dose treatments of zibotentan and dapagliflozin (Treatment C; Treatment A; Treatment B) in 3 treatment periods, separated by a washout period of at least 7 days between treatment periods.
Part 1Zibotentan (Treatment A)Participants will be administered with zibotentan once daily for 5 days.
Part 2: Treatment Sequence ABCZibotentan (Treatment A)Each participant will receive 3 single-dose treatments of zibotentan and dapagliflozin (Treatment A; Treatment B; Treatment C) in 3 treatment periods, separated by a washout period of at least 7 days between treatment periods.
Part 2: Treatment Sequence ABCDapagliflozin (Treatment A)Each participant will receive 3 single-dose treatments of zibotentan and dapagliflozin (Treatment A; Treatment B; Treatment C) in 3 treatment periods, separated by a washout period of at least 7 days between treatment periods.
Part 2: Treatment Sequence ABCZibotentan/Dapagliflozin - Formulation 1 (Treatment B)Each participant will receive 3 single-dose treatments of zibotentan and dapagliflozin (Treatment A; Treatment B; Treatment C) in 3 treatment periods, separated by a washout period of at least 7 days between treatment periods.
Part 2: Treatment Sequence ABCZibotentan/Dapagliflozin - Formulation 2 (Treatment C)Each participant will receive 3 single-dose treatments of zibotentan and dapagliflozin (Treatment A; Treatment B; Treatment C) in 3 treatment periods, separated by a washout period of at least 7 days between treatment periods.
Part 2: Treatment Sequence BCADapagliflozin (Treatment A)Each participant will receive 3 single-dose treatments of zibotentan and dapagliflozin (Treatment B; Treatment C; Treatment A) in 3 treatment periods, separated by a washout period of at least 7 days between treatment periods.
Part 2: Treatment Sequence BCAZibotentan/Dapagliflozin - Formulation 1 (Treatment B)Each participant will receive 3 single-dose treatments of zibotentan and dapagliflozin (Treatment B; Treatment C; Treatment A) in 3 treatment periods, separated by a washout period of at least 7 days between treatment periods.
Part 2: Treatment Sequence BCAZibotentan/Dapagliflozin - Formulation 2 (Treatment C)Each participant will receive 3 single-dose treatments of zibotentan and dapagliflozin (Treatment B; Treatment C; Treatment A) in 3 treatment periods, separated by a washout period of at least 7 days between treatment periods.
Part 2: Treatment Sequence CABDapagliflozin (Treatment A)Each participant will receive 3 single-dose treatments of zibotentan and dapagliflozin (Treatment C; Treatment A; Treatment B) in 3 treatment periods, separated by a washout period of at least 7 days between treatment periods.
Part 2: Treatment Sequence CABZibotentan/Dapagliflozin - Formulation 1 (Treatment B)Each participant will receive 3 single-dose treatments of zibotentan and dapagliflozin (Treatment C; Treatment A; Treatment B) in 3 treatment periods, separated by a washout period of at least 7 days between treatment periods.
Part 2: Treatment Sequence CABZibotentan/Dapagliflozin - Formulation 2 (Treatment C)Each participant will receive 3 single-dose treatments of zibotentan and dapagliflozin (Treatment C; Treatment A; Treatment B) in 3 treatment periods, separated by a washout period of at least 7 days between treatment periods.
Primary Outcome Measures
NameTimeMethod
Part 1: Metabolites in Safety Testing samplingDay 1 through Day 6 (pre-dose, 30 min; 1, 2, 4, 6, 8, 12 and 24 hours post dose)

Plasma sample will be collected to understand the PK profiling of zibotentan metabolites and to meet the regulatory requirements.

Part 2: Maximum observed plasma drug concentration (Cmax)Day 1 through Day 3 of each treatment period

Relative bioavailability of zibotentan and dapagliflozin after dosing with two different FDC formulations and dosing with separate formulations of zibotentan and dapagliflozin will be evaluated.

Part 2: Area under plasma concentration time curve from zero to infinity (AUCinf)Day 1 through Day 3 of each treatment period

Relative bioavailability of zibotentan and dapagliflozin after dosing with two different FDC formulations and dosing with separate formulations of zibotentan and dapagliflozin will be evaluated.

Part 2: Area under the plasma concentration curve from zero to the last quantifiable concentration (AUClast)Day 1 through Day 3 of each treatment period

Relative bioavailability of zibotentan and dapagliflozin after dosing with two different FDC formulations and dosing with separate formulations of zibotentan and dapagliflozin will be evaluated.

Part 2: Observed concentration at 24 hours post-dose (C24)Day 1 through Day 3 of each treatment period

Relative bioavailability of zibotentan and dapagliflozin after dosing with two different FDC formulations and dosing with separate formulations of zibotentan and dapagliflozin will be evaluated.

Secondary Outcome Measures
NameTimeMethod
Part 2: Time to reach peak or maximum observed concentration (tmax)Day 1 through Day 3 of each treatment period

Relative bioavailability of zibotentan and dapagliflozin after dosing with two different FDC formulations and dosing with separate formulations of zibotentan and dapagliflozin will be evaluated.

Part 2: Terminal rate constant (λz)Day 1 through Day 3 of each treatment period

Relative bioavailability of zibotentan and dapagliflozin after dosing with two different FDC formulations and dosing with separate formulations of zibotentan and dapagliflozin will be evaluated.

Part 2: Half life associated with λz (t½λz)Day 1 through Day 3 of each treatment period

Relative bioavailability of zibotentan and dapagliflozin after dosing with two different FDC formulations and dosing with separate formulations of zibotentan and dapagliflozin will be evaluated.

Part 2: Apparent total body clearance of drug from plasma after extravascular administration (CL/F)Day 1 through Day 3 of each treatment period

Relative bioavailability of zibotentan and dapagliflozin after dosing with two different FDC formulations and dosing with separate formulations of zibotentan and dapagliflozin will be evaluated.

Part 2: Volume of distribution at steady state following extravascular administration (Vz/F)Day 1 through Day 3 of each treatment period

Relative bioavailability of zibotentan and dapagliflozin after dosing with two different FDC formulations and dosing with separate formulations of zibotentan and dapagliflozin will be evaluated.

Part 1 and Part 2: Number of adverse events and serious adverse eventsFrom Sceerning to Follow-up Visit approximately 40 days for Part 1 and 49 days for Part 2

Safety and tolerability of zibotentan and dapagliflozin will be studied.

Trial Locations

Locations (1)

Research Site

🇺🇸

Brooklyn, Maryland, United States

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