miRNAs in Periodontal Disease

Completed

• Conditions: Periodontitis; Periodontal Diseases; Gingivitis
• Interventions: Diagnostic Test: Control; Diagnostic Test: Smokers with periodontally healthy; Diagnostic Test: Non-smokers with gingivitis; Diagnostic Test: Smokers with gingivitis; Diagnostic Test: Non-smokers with periodontitis; Diagnostic Test: Smokers with periodontitis

• Registration Number: NCT05046678
• Lead Sponsor: Bulent Ecevit University

Brief Summary

It has been stated that microRNA (miRNA) play an important role in development, homeostasis and immune functions, and abnormal miRNA expression may cause faster disease progression. The aim of this study was to determine miR-203, miR-142-3p, miR-146a, miR-146b, miR-155, miR-29b gene expressions in saliva of the patients with periodontal disease before and after non-surgical periodontal therapy (NSPT) and to evaluate the effect of smoking on these miRNAs. A total of 90 individuals, 30 with periodontitis, 30 with gingivitis, and 30 periodontally healthy (control group), were included. These three groups were divided into subgroups as smoking and non-smoking individuals, with 15 people in each group. NSPT was applied to patients with periodontitis and gingivitis. Saliva samples and clinical parameters were taken from at baseline and repeated 6 weeks after NSPT.

Detailed Description

Not available

Study Timeline

• Study Start: 2019-03-01
• Primary Completion: 2019-09-01
• Study Completion: 2020-11-01
• Status Verified: 2021-09
• Study First Submitted: 2021-09-13
• Study First Submitted QC: 2021-09-13
• Last Update Submitted: 2021-09-13
• Study First Posted: 2021-09-16
• Last Update Posted: 2021-09-16

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 90

Inclusion Criteria

Have at least 20 natural teeth, excluding third molars. Periodontitis patients had at least two non-adjacent sites per quadrant with probing depth (PD) ≥ 5 mm and clinical attachment level (CAL) ≥ 5 mm with gingival inflammation, and alveolar bone loss affecting >30% of the teeth, as detected on clinical and radiographical examinations.

Gingivitis patients had without attachment and alveolar bone loss who had gingival inflammation, PD ≤ 3 mm and BOP > 30% in the entire mouth Periodontally healthy control group had no sign of gingival inflammation, no PD > 3mm and no evidence of attachment or bone loss

Exclusion Criteria

History of systemic disease. Regular use of any drugs which can effect the immune system or inflammatory response in the 6 months preceding the start of the study.

Periodontal treatment during last 6 months that could affect periodontal status.

History of radiotherapy or chemotherapy. Current pregnancy, lactation or menopause.

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Arm && Interventions

Group	Intervention	Description
Smokers with periodontally healthy	Non-smokers with gingivitis	Periodontally healthy, smoking
Control	Smokers with gingivitis	Periodontally healthy, non-smoking
Smokers with periodontally healthy	Control	Periodontally healthy, smoking
Non-smokers with gingivitis	Smokers with gingivitis	Gingivitis, non-smoking
Control	Smokers with periodontally healthy	Periodontally healthy, non-smoking
Control	Non-smokers with gingivitis	Periodontally healthy, non-smoking
Control	Non-smokers with periodontitis	Periodontally healthy, non-smoking
Control	Smokers with periodontitis	Periodontally healthy, non-smoking
Smokers with periodontally healthy	Non-smokers with periodontitis	Periodontally healthy, smoking
Smokers with periodontally healthy	Smokers with gingivitis	Periodontally healthy, smoking
Non-smokers with gingivitis	Control	Gingivitis, non-smoking
Smokers with gingivitis	Control	Gingivitis, smoking
Smokers with gingivitis	Non-smokers with gingivitis	Gingivitis, smoking
Non-smokers with periodontitis	Control	Periodontitis, non-smoking
Smokers with periodontitis	Control	Periodontitis, smoking
Smokers with periodontitis	Smokers with gingivitis	Periodontitis, smoking
Smokers with periodontally healthy	Smokers with periodontitis	Periodontally healthy, smoking
Non-smokers with gingivitis	Smokers with periodontally healthy	Gingivitis, non-smoking
Non-smokers with gingivitis	Non-smokers with periodontitis	Gingivitis, non-smoking
Smokers with gingivitis	Smokers with periodontally healthy	Gingivitis, smoking
Smokers with gingivitis	Non-smokers with periodontitis	Gingivitis, smoking
Non-smokers with periodontitis	Smokers with periodontally healthy	Periodontitis, non-smoking
Smokers with periodontitis	Non-smokers with periodontitis	Periodontitis, smoking
Non-smokers with periodontitis	Non-smokers with gingivitis	Periodontitis, non-smoking
Non-smokers with periodontitis	Smokers with gingivitis	Periodontitis, non-smoking
Non-smokers with gingivitis	Smokers with periodontitis	Gingivitis, non-smoking
Non-smokers with periodontitis	Smokers with periodontitis	Periodontitis, non-smoking
Smokers with periodontitis	Smokers with periodontally healthy	Periodontitis, smoking
Smokers with periodontitis	Non-smokers with gingivitis	Periodontitis, smoking
Smokers with gingivitis	Smokers with periodontitis	Gingivitis, smoking

Primary Outcome Measures

Name	Time	Method
miRNAs gene expressions in the periodontal disease	baseline	miR-203, miR-142-3p, miR-146a, miR-146b, miR-155, miR-29b gene expressions in saliva

Secondary Outcome Measures

Name	Time	Method
miRNAs gene expressions in the periodontal disease	baseline and 6 weeks after treatment	miR-203, miR-142-3p, miR-146a, miR-146b, miR-155, miR-29b gene expressions in saliva before and after non-surgical periodontal treatment

Trial Locations

Locations (1)

Ordu University; 🇹🇷 Ordu, In The USA Or Canada, Please Select..., Turkey