Investigation of the human gut microbiota pre-, during and post long-term stay in sub-Saharan Africa

Recruiting

Conditions: Changes in gut microbiota after dietary changes due to an overseas stay

Registration Number: DRKS00034691

Lead Sponsor: ZIEL - Institut for Food and Health der Technischen Universität München

Brief Summary: Not available

Detailed Description: Not available

Recruitment & Eligibility

Status: Recruiting

Sex: All

Target Recruitment: 43

Inclusion Criteria

• Men / Women, aged 18-28 years (as specified by the weltwärts program)
• Residence in Germany
• Participation in the weltwärts program with a planned 12-month stay in Uganda

Exclusion Criteria

• Not residing in Germany within the last 5 years
• Pregnancy
• Chronic gastrointestinal diseases (chronic inflammatory bowel diseases, colon cancer, diverticulitis)
• Use of antibiotics in the last 4 weeks and/or regularly during the stay abroad (exception: medications for malaria prophylaxis)
• Regular use of probiotics (discontinuation at least 2 weeks before examination T0)
• Acute gastrointestinal illness (diarrhea)
• Family history of chronic intestinal diseases

Study & Design

Study Type: observational

Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
• Levels of short-chain fatty acids in stool, particularly butyrate<br>• Composition and diversity (i.e., alpha- and beta-diversity) of gut microbiota in stool

Secondary Outcome Measures

Name	Time	Method
• Stool weight<br>• Stool consistency<br>• Stool transit time<br>• Bile acids in stool<br>• Functional microbial genes in stool<br>• Presence of specific bacteria and bacteriophages in stool<br>• Frequency of food consumption<br>• Anthropometric data (weight, height, waist and hip circumference, age, sex, gastrointestinal medical history, vaccinations)<br>• Resting energy expenditure<br>• Body composition<br>• H2/CH4 breath gas analysis<br>• Additional microbiota composition (saliva, skin)<br>• Micronutrients in blood<br>• Trimethylamine-N-oxide (TMAO) in blood