Assessment of Candidate Protein Expression in Human Breast Cancer Specimens
Overview
- Phase
- Not Applicable
- Intervention
- Not specified
- Conditions
- Breast Cancer
- Sponsor
- Case Comprehensive Cancer Center
- Enrollment
- 460
- Locations
- 1
- Primary Endpoint
- Differential expression of candidate genes between normal and cancer breast tissues
- Status
- Completed
- Last Updated
- 3 years ago
Overview
Brief Summary
RATIONALE: Studying the genes expressed in samples of tumor tissue from patients with cancer may help doctors identify biomarkers related to cancer.
PURPOSE: This research study is looking at tumor tissue samples from patients who have undergone surgery for breast cancer.
Detailed Description
OBJECTIVES: * To determine if candidate genes display differential expression between normal and cancer breast tissues. OUTLINE: Previously collected breast cancer tissue samples (paraffin embedded blocks) and normal adjacent tissue are used for immunohistochemistry studies of expression of candidate genes.
Investigators
Eligibility Criteria
Inclusion Criteria
- •DISEASE CHARACTERISTICS:
- •Diagnosis of breast cancer
- •Tissue samples available
- •Samples are selected based on presence or absence of tumor receptors, (i.e.; estrogen, progesterone, and HER2 receptors) and grouped into following 4 categories:
- •Estrogen receptor (ER)-positive/progesterone receptor (PR)-positive/HER2-negative
- •ER-positive/PR-positive/ HER2-positive by FISH
- •ER-negative/PR-negative/HER2-positive
- •ER-negative/PR-negative/HER2-negative(triple negative or basal-type cancers)
- •PATIENT CHARACTERISTICS:
- •Menopausal status not specified
Exclusion Criteria
- •There are no exclusion criteria in this protocol
Outcomes
Primary Outcomes
Differential expression of candidate genes between normal and cancer breast tissues
Time Frame: non-applicable
Approximately 5 slides of each tumor will be collected to provide sufficient samples for replicate staining, if necessary, as well as a staining for a positive control