Examining the Impact of Blood Flow Restricted Aerobic Exercise on PGC-1α mRNA Expression in Young Healthy Males
- Conditions
- Mitochondrial Biogenesis
- Interventions
- Other: BFR ExerciseOther: CTL Exercise
- Registration Number
- NCT03717948
- Lead Sponsor
- Queen's University
- Brief Summary
This crossover study investigates the effects of restricting arterial blood flow during cycling exercise on PGC-1α and its upstream signalling pathways. In a randomized order, participants will exercise once for 30 minutes with restricted blood flow and then will exercise once for 30 minutes with no blood flow restriction.
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- COMPLETED
- Sex
- Male
- Target Recruitment
- 13
- 3 hours of physical activity per week
- No previous cycling training
- No concurrent involvement in another training program
- Body mass index < 30 kg/m2
- Presence of cardiovascular disease
- Presence of metabolic disease
- Taking regular oral medication
- Current smoker
Study & Design
- Study Type
- INTERVENTIONAL
- Study Design
- CROSSOVER
- Arm && Interventions
Group Intervention Description CTL - BFR CTL Exercise CTL Exercise then BFR Exercise CTL - BFR BFR Exercise CTL Exercise then BFR Exercise BFR - CTL CTL Exercise BFR Exercise then CTL Exercise BFR - CTL BFR Exercise BFR Exercise then CTL Exercise
- Primary Outcome Measures
Name Time Method Peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) messenger ribonucleic acid (mRNA) 15 minutes before exercise and 3 hours post-exercise for both exercise sessions Fold-change in PGC-1α mRNA expression
- Secondary Outcome Measures
Name Time Method Catecholamine concentrations 15 minutes before exercise and immediately post-exercise for both exercise sessions Catecholamine concentrations in venous blood
Total and phosphorylated protein kinase A (PKA) 15 minutes before exercise and immediately post-exercise for both exercise sessions Fold change in PKA phosphorylation
Total and phosphorylated acetyl coenzyme A carboxylase (ACC) 15 minutes before exercise and immediately post-exercise for both exercise sessions Fold change in ACC phosphorylation
Heart rate (HR) During both exercise sessions Average HR between conditions
Total and phosphorylated p38 mitogen-activated protein kinase (MAPK) 15 minutes before exercise and immediately post-exercise for both exercise sessions Fold change in p38 MAPK phosphorylation
Blood lactate During both exercise sessions (lactate); 15 minutes before and immediately after both exercise sessions Average \[lactate\] between conditions
Oxygenated and deoxygenated hemoglobin During both exercise sessions and up to 10 minutes after (for cuff occlusion) Average oxygenated and deoxygenated hemoglobin as a percentage of same day max values
Acetylated p53 15 minutes before exercise and immediately post-exercise for both exercise sessions Fold change in acetylated p53
Revolutions per minute (RPM) During both exercise sessions Average RPM between conditions
Ratings of perceived exertion (RPE) During both exercise sessions Average RPE between conditions
Trial Locations
- Locations (1)
Queen's University
🇨🇦Kingston, Ontario, Canada