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Investigation of the Impact of Concomitant Use of Multiple Doses of Clopidogrel With Multiple Doses of Dabigatran Etexilate on the Pharmacokinetic and Pharmacodynamic Parameters in Healthy Male Subjects

Phase 1
Completed
Conditions
Healthy
Interventions
Registration Number
NCT02171598
Lead Sponsor
Boehringer Ingelheim
Brief Summary

The primary objective of the study (Main Part 2) was to investigate whether and to which extent a combination of multiple oral doses (steady state conditions) of 75 mg of clopidogrel q.d. (after a loading dose of 300 mg) and multiple doses of 150 mg of dabigatran etexilate b.i.d. at steady state affects pharmacokinetic and pharmacodynamic parameters of dabigatran etexilate and clopidogrel.

The objective of the preceding Pilot Part 1 of the study was to explore the effect of a single dose of 300 mg clopidogrel administered after multiple doses of 75 mg and 150 mg dabigatran had reached steady state, regarding safety as well as pharmacokinetic and pharmacodynamic parameters.

The Main Part 3 of the study moreover was to compare intra-individually the effects of a single dose of 600 mg clopidogrel with the same dose, 600 mg clopidogrel, given additionally to multiple doses of 150 mg dabigatran in steady state condition with respect to safety and pharmacokinetic and pharmacodynamic parameters.

Detailed Description

Not available

Recruitment & Eligibility

Status
COMPLETED
Sex
Male
Target Recruitment
44
Inclusion Criteria
  1. Healthy male subjects according to the following criteria: Based upon a complete medical history, including the physical examination, vital signs (blood pressure, pulse rate), body temperature, 12-lead electrocardiogram, clinical laboratory tests
  2. Age ≥18 and Age ≤40 years
  3. Body mass index (BMI) ≥18.5 and ≤29.9 kg/m2 (Body Mass Index)
  4. Signed and dated written informed consent prior to admission to the study in accordance with GCP and the local legislation.
Exclusion Criteria
  1. Any gastrointestinal, hepatic, renal, respiratory, cardiovascular, metabolic, immunological or hormonal disorders

  2. Subjects who in the investigator's judgement are perceived as having an increased risk of bleeding, for example because of:

    • Hemorrhagic disorders or bleeding diathesis
    • Occult blood in faeces or haematuria
    • Trauma or surgery within the last month or as long as an excessive risk of bleeding persists after these events, or planned surgery during trial participation
    • History of arteriovenous malformation or aneurysm
    • History of gastroduodenal ulcer disease, gastrointestinal haemorrhage and haemorrhoids
    • History of intracranial, intraocular, spinal, retroperitoneal, or atraumatic intraarticular bleeding
    • Use of drugs that may interfere with haemostasis during trial conduct (e.g.acetyl salicylic acid or other non-steroidal anti-inflammatory drugs)
  3. Relevant surgery of gastrointestinal tract

  4. Diseases of the central nervous system (such as epilepsy) or psychiatric disorders or neurological disorders

  5. History of relevant orthostatic hypotension, fainting spells or blackouts

  6. Chronic or relevant acute infections

  7. History of allergy/hypersensitivity (including drug allergy) which is deemed relevant to the trial as judged by the investigator

  8. Intake of any medication within four weeks of first dosing

  9. Use of drugs which might reasonably influence the results of the trial based on the knowledge at the time of protocol preparation within four weeks prior to administration or during the trial, especially inhibitors or inducers of P-gp, CYP3A4 CYP2C9 or CYP2C19

  10. Intake of medication, which influences the blood clotting, i.e., acetylsalicylic acid, nonsteroidal anti-rheumatic drugs, cumarin etc. within 10 days prior to administration or during the trial

  11. Participation in another trial with an investigational drug within one month prior to administration or during the trial

  12. Alcohol abuse (more than 60 g/day on a regular basis)

  13. Drug abuse

  14. Within 5 days of study medication no intake of grapefruit, grapefruit juice, or products containing grapefruit juice, Seville oranges, garlic supplements, or St. John's Worth

  15. Blood donation (more than 100 mL within four weeks prior to administration)

  16. Excessive physical activities (within one week prior to administration or during the trial)

  17. Any laboratory value outside the reference range that is of clinical relevance

  18. Inability to comply with dietary regimen of study centre

  19. Male subjects do not agree to minimise the risk of female partners becoming pregnant from the first dosing day until 3 months after the completion of the study. Acceptable methods of contraception comprises barrier contraception and a medically accepted contraceptive method for the female partner (intra-uterine device with spermicide, hormonal contraceptive since at least two months)

Study & Design

Study Type
INTERVENTIONAL
Study Design
CROSSOVER
Arm && Interventions
GroupInterventionDescription
CrossoverDabigatran low doseclopidogrel + dabigatran / clopidogrel / dabigatran in randomized order
CrossoverDabigatran high doseclopidogrel + dabigatran / clopidogrel / dabigatran in randomized order
Fixed sequence 2Dabigatran high doseintra-individual comparison with the fixed sequence of a single dose of 600mg clopidogrel alone and the combination of dabigatran 150 mg in steady state plus single dose of 600 mg clopidogrel
Fixed sequence 1Clopidogrelmultiple-dose, fixed-sequence with 2 periods of 4 days separated by a washout period of at least 14 days. A single dose of 300 mg clopidogrel will be given on top of 75 mg or 150 mg dabigatran in steady state.
Fixed sequence 1Dabigatran high dosemultiple-dose, fixed-sequence with 2 periods of 4 days separated by a washout period of at least 14 days. A single dose of 300 mg clopidogrel will be given on top of 75 mg or 150 mg dabigatran in steady state.
Fixed sequence 1Dabigatran low dosemultiple-dose, fixed-sequence with 2 periods of 4 days separated by a washout period of at least 14 days. A single dose of 300 mg clopidogrel will be given on top of 75 mg or 150 mg dabigatran in steady state.
CrossoverClopidogrelclopidogrel + dabigatran / clopidogrel / dabigatran in randomized order
Fixed sequence 2Clopidogrelintra-individual comparison with the fixed sequence of a single dose of 600mg clopidogrel alone and the combination of dabigatran 150 mg in steady state plus single dose of 600 mg clopidogrel
Primary Outcome Measures
NameTimeMethod
Cmax,ss (maximum measured concentration of the analyte in plasma at steady state)up to 19 days

for total dabigatran, clopidogrel and the inactive metabolite SR26334

AUECIPA,0-24 (area under the effect curve of inhibition of platelet aggregation after the first dose of clopidogrel)up to 19 days
AUCτ,ss (area under the concentration-time curve of the analyte in plasma over one dosing interval at steady state)up to 19 days

for total dabigatran, clopidogrel and the inactive metabolite SR26334

AUC0-24,1 (area under the concentration-time curve of the analyte in plasma over one dosing interval after the loading dose)up to 19 days

for clopidogrel and the inactive metabolite SR26334

Cmax,1 (maximum measured concentration of the analyte in plasma after the loading dose)up to 19 days

for total dabigatran, clopidogrel and the inactive metabolite SR26334

Emax,IPA,0-24 (maximum percentage change - compared to baseline - in adenosine diphosphate-induced platelet aggregation after the loading dose of clopidogrel)baseline, up to 19 days
Secondary Outcome Measures
NameTimeMethod
tmax (time from dosing to the maximum concentration of the analyte in plasma)up to 8 weeks

Clopidogrel and SR 26334 after the loading dose

AUC0-infinity (area under the concentration-time curve of the analyte in plasma over the time interval from 0 extrapolated to infinity)up to 8 weeks

Clopidogrel and SR 26334 after the loading dose

CL/F (apparent clearance of the analyte in the plasma after extravascular administration)up to 8 weeks

Clopidogrel and SR 26334 after the loading dose

Cmax, ssup to 8 weeks

free dabigatran after multiple dosing

AUCτ,ssup to 8 weeks

free dabigatran after multiple dosing

AUC0-tz,ss (area under the concentration-time curve of the analyte in plasma from the time point 0 after the last dose at steady state to the last quantifiable analyte plasma concentration within the uniform dosing interval τ)up to 8 weeks

Clopidogrel and SR 26334; dabigatran (free and total) after multiple dosing

tmax,ss (time from last dosing to the maximum concentration of the analyte in plasma at steady state on day 4)up to 8 weeks

Clopidogrel and SR 26334; dabigatran (free and total) after multiple dosing

Cmin,ss (minimum measured concentration of the analyte in plasma at steady state over a uniform dosing interval τ)up to 8 weeks

Clopidogrel and SR 26334; dabigatran (free and total) after multiple dosing

MRTpo,ss (mean residence time of the analyte in the body at steady state after oral administration)up to 8 weeks

Clopidogrel and SR 26334; dabigatran (free and total) after multiple dosing

Vz/F,ss (apparent volume of distribution during the terminal phase λz at steady state following an extravascular administration)up to 8 weeks

Clopidogrel and SR 26334; dabigatran (free and total) after multiple dosing

PTF (peak trough fluctuation)up to 8 weeks

Clopidogrel and SR 26334; dabigatran (free and total) after multiple dosing

Cavg (average concentration of the analyte in plasma at steady state over a uniform dosing interval)up to 8 weeks

Clopidogrel and SR 26334; dabigatran (free and total) after multiple dosing

AUECt1-t2 (area under the effect curve (baseline corrected by ratio))up to 8 weeks

for activated partial thromboplastin time, thrombin time and ecarin clotting time

AUC0-tz (area under the concentration-time curve of the analyte in plasma from the time point 0 to the last quantifiable analyte plasma concentration)up to 8 weeks

Clopidogrel and SR 26334 after the loading dose

λz (terminal rate constant in plasma)up to 8 weeks

Clopidogrel and SR 26334 after the loading dose

t1/2 (terminal half-life of the analyte in plasma)up to 8 weeks

Clopidogrel and SR 26334 after the loading dose

tz,ss (time of last measureable concentration of the analyte in plasma within the dosing interval τ at steady state)up to 8 weeks

Clopidogrel and SR 26334; dabigatran (free and total) after multiple dosing

CL/F,ss (apparent clearance of the analyte in the plasma at steady state after extravascular multiple dose administration)up to 8 weeks

Clopidogrel and SR 26334; dabigatran (free and total) after multiple dosing

MRTpo (mean residence time of the analyte in the body after oral administration)up to 8 weeks

Clopidogrel and SR 26334 after the loading dose

Vz/F (apparent volume of distribution during the terminal phase λz following an extravascular dose)up to 8 weeks

Clopidogrel and SR 26334 after the loading dose

Cpre,ss (predose concentration of the analyte in plasma at steady state immediately before administration of the next dose)up to 8 weeks

Clopidogrel and SR 26334; dabigatran (free and total) after multiple dosing

tmin,ss (time from last dosing to the minimum concentration of the analyte in plasma at steady state over a uniform dosing interval τ)up to 8 weeks

Clopidogrel and SR 26334; dabigatran (free and total) after multiple dosing

ERmax (maximum effect ratio)up to 8 weeks

for activated partial thromboplastin time, thrombin time and ecarin clotting time

tmax (time to maximum effect)up to 8 weeks

for activated partial thromboplastin time, thrombin time and ecarin clotting time

Occurence of Adverse Eventsup to 13 weeks
Assessment of Tolerability by investigatorup to 13 weeks
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