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A Study of AL-794 to Evaluate the Safety, Tolerability, and Pharmacokinetics of Single and Multiple Doses, and the Antiviral Activity of Multiple Doses in an Influenza Challenge Study

Phase 1
Completed
Conditions
Influenza
Interventions
Other: Placebo/Vehicle
Registration Number
NCT02588521
Lead Sponsor
Alios Biopharma Inc.
Brief Summary

This randomized, double-blind, placebo-controlled, 4 part study will assess the safety, tolerability, pharmacokinetics and antiviral activity of orally administered AL-794 in healthy volunteers (HV).

Detailed Description

Not available

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
152
Inclusion Criteria
  1. Subject has provided written consent.
  2. In the investigator's opinion, the subject is able to understand and comply with protocol requirements, instructions, and protocol stated restrictions and is likely to complete the study as planned.
  3. Subject is in good health as deemed by the investigator, based on the findings of a medical evaluation including medical history, physical examination, laboratory tests and ECG.
  4. Male or female, 18-60 years of age (Parts 1-3). For Part 4, Males or female, 18-55 years of age.
  5. Body mass index (BMI) 18-30kg/m2, inclusive. The minimum weight is 50 kg.
  6. A female subject is eligible to participate in this study if she is of non childbearing potential or postmenopausal and not receiving hormone replacement therapy.
  7. If male, subject is surgically sterile or practicing specific forms of birth control until 90 days after the end of the study.
Exclusion Criteria
  1. Clinically significant cardiovascular, respiratory, renal, gastrointestinal, hepatic, hematologic, neurologic, thyroid or any other medical illness or psychiatric disorder, as determined by the Investigator, and/or Sponsor's Medical Monitor.

  2. Positive screening test for hepatitis A, B, C or HIV infection.

  3. Clinically significant laboratory abnormalities or abnormalities which are deemed to interfere with the ability to interpret study data.

  4. Creatinine clearance of less than 60 mL/min (Cockroft Gault).

  5. Total bilirubin, ALT, AST, or Alkaline Phosphatase > 1.2 X ULN.

  6. Any condition that, in the opinion of the investigator, would compromise the study's objectives or the well-being of the subject or prevent the subject from meeting the study requirements.

  7. Participation in an investigational drug trial or having received an investigational vaccine within 3 months prior to study medication (or inoculation for Part 4 subjects).

  8. Clinically significant abnormal ECG findings.

  9. Clinically significant blood loss or elective blood donation of significant volume (i.e., > 500 mL) within 90 days of first dose of study drug

  10. Heart rate, respiratory rate, temperature or blood pressure values outside of the normal range, per local standards.

  11. Unwilling to abstain from alcohol for 1 week before the start of admission until the final Completion Visit assessments.

  12. History of regular alcohol intake > 14 units per week of alcohol for females and > 21 units per week for males (one unit is defined as 8 g alcohol) within 3 months of admission.

  13. For Parts 1-3, subjects with a history of tobacco use or use of nicotine-containing products within 2 weeks of the screening visit. For Part 4, subjects who have a significant history of any tobacco use at any time.

  14. The subject has a positive pre-study drug screen.

  15. The use of concomitant medications, including prescription, over the counter medications, herbal medications, inducers or inhibitors of CYP450 enzymes or drug transporters (including P-gp), within 14 days prior to the first dose of study medication, unless approved by the Sponsor's Medical Monitor. Occasional use of ibuprofen or acetaminophen is permitted.

  16. Exposure to more than four new investigational entities within 12 months prior to the first dosing day (Parts 1-3) or inoculation (Part 4).

  17. Evidence of active infection, including respiratory tract infection within 2 weeks prior to admission.

  18. Pregnant or nursing females, or women of childbearing potential. Men whose female partners are pregnant or contemplating pregnancy from the date of screening until 90 days after end of study.

  19. Hypersensitivity to the active substances or to any of the excipients of AL-794.

  20. Unwillingness or inability to comply with the study protocol for any other reason.

  21. Part 4 ONLY: Contraindications to challenge with influenza virus or procedures related or influenza challenge study. For example:

    • Subject is not sero-suitable.
    • Immunocompromised status or diagnosis of active autoimmune disease.
    • Known allergy to constituents of challenge virus.
    • History of severe illness during adulthood due to a respiratory virus.
    • Inability to tolerate repeated nasopharyngeal swabs.
    • Abnormal pulmonary function in the opinion of the Investigator, as evidenced by the responses to the respiratory screening questions and /or clinically significant abnormalities in spirometry.
    • Significant history of asthma.
    • Any significant abnormality altering the anatomy of the nose or nasopharynx.
    • Any clinically significant history of epistaxis.
    • Any nasal or sinus surgery within six months of inoculation.
    • Health care workers who are reasonably likely to come into contact with severely immuno-compromised patients
    • Presence of household member or close contact who has known immunodeficiency, is receiving immunosuppressant medication, is undergoing or soon to undergo cancer chemotherapy, has been diagnosed with emphysema, COPD, or other severe lung disease, has received a bone marrow or solid organ transplant, resides in a nursing home
    • Evidence of vaccinations within the four weeks prior to inoculation/dosing, whichever occurs first.
    • Intention to receive any vaccination(s) before the Day 28 Follow Up Visit
    • Prior participation in another Human Viral Challenge Study in the preceding 12 months taken from the date of inoculation in the previous study to the date of expected inoculation in this study.
    • Receipt of systemic glucocorticoids or systemic antiviral drugs within six months prior to inoculation/dosing.
    • Receipt of any systemic chemotherapy agent, immunoglobulins or any other cytotoxic or immunosuppressive drugs at any time.

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
AL-794AL-794AL-794 administered orally in a suspension
VehiclePlacebo/VehicleSuspension vehicle alone
Primary Outcome Measures
NameTimeMethod
Safety Data including but not limited to tabulation of adverse events, physical exam, vital signs, 12-lead ECGs and clinical lab resultsScreening to Day 28 in the Viral Challenge portion of the study.

Safety data including but not limited to tabulation of adverse events, physical exam, vital signs, 12-lead ECGs and clinical lab results (including chemistry, hematology, and urinalysis).

Secondary Outcome Measures
NameTimeMethod
Peak influenza viral load after treatmentinoculation to Day 28

Peak influenza viral load after treatment onset, as determined by quantitative PCR assay of nasopharyngeal swab

AUC: AL-794From baseline to Day 17 for Multiple Ascending Dose cohorts

Pharmacokinetic parameter of ALS-033719 and ALS-033927 (and other metabolites, if applicable) in plasma following multiple dose administration

Cmax: AL-794From baseline to Day 17 for Multiple Ascending Dose Cohorts

Pharmacokinetic parameter of ALS-033719 and ALS-033927 (and other metabolites, if applicable) in plasma following multiple dose administration

CMax: Fed vs. FastedFirst dose to Day 8 (Single Ascending Dose/Food Effect)

Comparison of Cmax after a single oral dose in HV in fasted conditions as compared with fed conditions.

AUC: Fed vs. FastedFirst dose to Day 8 (Single Ascending Dose/Food Effect)

Comparison of AUC after a single oral dose in HV in fasted conditions as compared with fed conditions.

Influenza Viral LoadBaseline to Day 28

AUC0-t of influenza viral load, as determined by quantitative polymerase chain reaction (PCR) assay of nasopharyngeal swab, from baseline (i.e., immediately prior to treatment onset) through checkout, Day 17, and completion of study

Trial Locations

Locations (1)

Hammersmith Medicines Research Ltd (HMR)

🇬🇧

London, United Kingdom

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