Expandability of Aging Adipose Tissue

Not Applicable

Completed

• Conditions: Obesity; Aging
• Interventions: Other: Overfeeding

• Registration Number: NCT02456870
• Lead Sponsor: University of Colorado, Denver

Brief Summary

The purpose of this pilot study is to determine whether adipose tissue loses some of its ability to expand in response to overfeeding in older compared to younger men. The investigators hypothesize that the adipose tissue response to overfeeding may be weakened in older when compared to younger men, thereby leading to worsened ectopic storage depots for older men.

Detailed Description

This pilot study will begin to address whether adipose tissue biology changes with advancing age. Specifically, we will test whether adipose tissue loses some of its ability to expand in response to overfeeding in older compared to younger men. To test this possibility the Investigators will recruit overweight and mildly obese older (age 55-75y) and younger (age 25-40y) men and collect adipose tissue biopsies before and after 7 days of overfeeding. The investigators will test how well the cells in adipose tissue expand in response to overfeeding and whether this differs between younger and older men.

Study Timeline

• Study First Submitted: 2015-04-08
• Study First Submitted QC: 2015-05-26
• Study First Posted: 2015-05-29
• Study Start: 2015-06
• Primary Completion: 2016-12
• Study Completion: 2018-12
• Status Verified: 2019-06
• Last Update Submitted: 2019-06-11
• Last Update Posted: 2019-06-13

Recruitment & Eligibility

• Status: COMPLETED
• Sex: Male
• Target Recruitment: 20

Inclusion Criteria

• Men
• Aged 25-40y OR 55-75y
• BMI 28-35 kg/m2
• Weight stable (±2kg over past 3 mo)
• Sedentary to moderately active (<3 d/wk of exercise lasting >20min)
• Non-smokers (never used or quit >7yrs prior)

Exclusion Criteria

• Women
• Currently using hormones, glucose or lipid lowering medication
• Engaged in exercise or diet program
• Currently gaining or losing weight or using weight loss drugs
• Diabetes mellitus (diagnosis, treatment, FBG ≥126 mg/dL)
• Uncontrolled metabolic disorders (e.g., thyroid)

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: SINGLE_GROUP

Arm && Interventions

Group	Intervention	Description
MA	Overfeeding	Middle-aged overweight and obese men (age 25-40yr)
OA	Overfeeding	Older overweight and obese men (age 55-75yr)

Primary Outcome Measures

Name	Time	Method
Change in adipose-derived stem cell (ASC) differentiation (Delta%oil red O+)	1 wk of overfeeding	age-related difference in the ASC differentiation response to overfeeding

Secondary Outcome Measures

Name	Time	Method
Change in ASC proliferation (Delta rate of cell growth)	1 wk of overfeeding	age-related difference in the ASC proliferative response to overfeeding
Change in expression of C/EBPalpha, C/EBPbeta, PPARgamma2, SREBP1c, SREBf1a, and Wnt10b (fold-changes in mRNA expression)	1 wk overfeeding	age-related difference in the pro-adipogenic response to overfeeding
Change in intramyocellular (IMCL) di-saturated diacylglycerol (DAG) localization (Delta %membrane:cytosol)	1 week overfeeding	age-related difference in the IMCL DAG response to overfeeding

Trial Locations

Locations (1)

Univeristy of Colorado, Anschutz Medical Campus; 🇺🇸 Aurora, Colorado, United States