Role of regulatory T cells in the pathogenesis of atopic dermatitis and psoriasis vulgaris.
- Conditions
- atopic dermatitiseczemapsoriasis10014982
- Registration Number
- NL-OMON33107
- Lead Sponsor
- niversitair Medisch Centrum Utrecht
- Brief Summary
Not available
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- Withdrawn
- Sex
- Not specified
- Target Recruitment
- 84
Atopic dermatitis patients:
- Adult (18-70 years) male or female patients diagnosed with atopic dermatitis
- Biopsy location (4x4 cm) should not be treated with topical steroids, dithranol or vitamin D3 preparations for at least 1 week;Psoriasis vulgaris patients:
- Adult (18-70 years) male or female patients diagnosed with psoriasis vulgaris
- Biopsy location (4x4 cm) should not be treated with topical steroids, dithranol or vitamin D3 preparations for at least 1 week;Healthy controls:
- Adult (18-70 years) male or female volunteers without a history of skin diseases
- Biopsy location (4x4 cm) should not be treated with topical steroids, dithranol or vitamin D3 preparations for at least 1 week
- No history of asthma and/or hay fever and/or house dust mite allergy;Patients undergoing plastic surgery:
- Adult (18-70 years of age) male or female patients without a history of skin diseases
- No history of asthma and/or hay fever and/or house dust mite allergy
- Use of systemic immunosuppressive drugs (i.e., cyclosporin, prednisolone, methotrexate, neotigasone, fumaric acid) in the 6 weeks prior to inclusion
- Exposure of biopsy location to (extraordinary) UV sunlight (i.e. UV-therapy, sunny holiday) in the weeks prior to inclusion
- (Secondary) skin infection
Study & Design
- Study Type
- Observational invasive
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method <p>Suppressive capacity of Tregs measured as suppression of proliferation by<br /><br>effector T cells (Teffs)<br /><br>(Tregs and Teffs will be co-cultured in the presence of antigen presenting<br /><br>cells. Teffs will be labeled with CFSE. Dilution of CFSE is a measure for<br /><br>proliferation) </p><br>
- Secondary Outcome Measures
Name Time Method <p>not applicable</p><br>