Muscle Response to High Intensity Interval Resistance Training (HIIRT)

Not Applicable

Completed

• Conditions: Exercise; Resistance Training
• Interventions: Other: Traditional Resistance Training; Other: High Intensity Interval Resistance Training

• Registration Number: NCT04321772
• Lead Sponsor: University of Padova

Brief Summary

Resistance training (RT) is one of the most important stimuli for muscle hypertrophy and it plays an important role on weight loss and fatty acid oxidation increase. RT affects anabolic pathways but the differences among various training techniques has been till now were poorly investigated. The aim of this study was to compare the effect of two different intensity of training, high-intensity interval resistance training (HIIRT) and traditional resistance training (TRT), on muscle signalling pathway.

Detailed Description

Nine young healthy subjects performed HIIRT and TRT protocol in two different moments and with different legs. HIIRT technique consisted of three sets of: 6 repetitions at 6RM (6 repetitions maximum) and then 20 seconds of rest and 2/3 repetitions (until exhaustion) repeated for 3 times with 2'30" rest between sets; while TRT consisted of 3 sets of 15 reps with 75 sec of rest between sets. Biopsies from the vastus lateralis were taken at baseline (pre), immediately (0h) at the end of training, 6 hours (6h) and 24 hours (24h) after training. Western blot and RT-PCR mRNA (Reverse transcriptase-polymerase chain reaction) analysis were performed to assess muscle signalling pathway activation

Study Timeline

• Study Start: 2012-02-01
• Primary Completion: 2012-05-15
• Study Completion: 2013-02-10
• Status Verified: 2020-03
• Study First Submitted: 2020-03-19
• Study First Submitted QC: 2020-03-24
• Last Update Submitted: 2020-03-24
• Study First Posted: 2020-03-25
• Last Update Posted: 2020-03-25

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 9

Inclusion Criteria

• moderate active
• experience with resistance training

Exclusion Criteria

• history of recent myocardial infarction,
• severe cardiac arrhythmia,
• unstable angina,
• poorly controlled hypertension,
• poorly controlled diabetes mellitus,
• frequent or complex ventricular ectopy which might interfere with one's ability to adhere to exercise protocols

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: CROSSOVER

Arm && Interventions

Group	Intervention	Description
Traditional Resistance Training (TRT)	Traditional Resistance Training	TRT protocol performed 3 series of 15 repetitions at 60% 1RM with 75" of rest between sets.
High Intensity Interval Resistance Training (HIIRT)	High Intensity Interval Resistance Training	HIIRT technique consisted of three sets of: 6 repetitions at 80% 1RM (1 repetition maximum) and then 20 seconds of rest and 2/3 repetitions (until exhaustion) repeated for 3 times with 2'30" rest between sets; while TRT consisted of 3 sets of 15 reps with 75 sec of rest between sets.

Primary Outcome Measures

Name	Time	Method
IGF-1 mRNA (messenger ribonucleic acid) in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)	24 hours	IGF-1 mRNA in skeletal muscle tissue taken by biopsy
pERK 1/2Thr202/Tyr204 in skeletal muscle tissue measured by immunoblot	24 hours	measurement of pERK 1/2Thr202/Tyr204 in skeletal muscle tissue taken by biopsy
pS6Ser235/236 in skeletal muscle tissue measured by immunoblot	24 hours	measurement of pS6Ser235/236 in skeletal muscle tissue taken by biopsy
p4EBP1Thr37/46 in skeletal muscle tissue measured by immunoblot	24 hours	measurement of p4EBP1Thr37/46 in skeletal muscle tissue taken by biopsy
ACC (acetyl-coenzyme A carboxylase) phosphorylation in skeletal muscle tissue measured by immunoblot	24 hours	ACC phosphorylation in skeletal muscle tissue taken by biopsy
pAMPKThr172 in skeletal muscle tissue measured by immunoblot	24 hours	measurement of pAMPKThr172 in skeletal muscle tissue taken by biopsy
IGF-1ea (insulin like growth factor) mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)	24 hoursn	IGF-1ea mRNA in skeletal muscle tissue taken by biopsy
MGF (mechanical growth factor) mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)	24 hours	MGF mRNA in skeletal muscle tissue taken by biopsy
STARS mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)	24 hoursn	STARS mRNA in skeletal muscle tissue taken by biopsy
Myogenin mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)	24 hours	Myogenin mRNA in skeletal muscle tissue taken by biopsy
IL-6 (interleukin 6) mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)	24 hours	IL-6 mRNA in skeletal muscle tissue taken by biopsy
PGC1a4 (Peroxisome proliferator-activated receptor gamma coactivator 1-alpha 4) mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)	24 hours	PGC1a4 mRNA in skeletal muscle tissue taken by biopsy
PGC1a (Peroxisome proliferator-activated receptor gamma coactivator 1-alpha) mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)	24 hours	PGC1a mRNA in skeletal muscle tissue taken by biopsy
Atrogin mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)	24 hours	Atrogin mRNA in skeletal muscle tissue taken by biopsy
Beclin mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)	24 hours	Beclin mRNA in skeletal muscle tissue taken by biopsy
Myostatin mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)	24 hours	Myostatin mRNA in skeletal muscle tissue taken by biopsy

Trial Locations

Locations (1)

Nutrition and Exercise Lab, DSB, University of Padova; 🇮🇹 Padova, Italy