Effects of de-alcoholised wine on Deoxyribonucleic Acid (DNA) damage.
Not Applicable
Completed
- Conditions
- Prevention of spontaneous and oxidative damage to DNA in healthy womenDiet and Nutrition - Other diet and nutrition disorders
- Registration Number
- ACTRN12609000013268
- Lead Sponsor
- Australian Vintage Ltd
- Brief Summary
Not available
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- Completed
- Sex
- Female
- Target Recruitment
- 8
Inclusion Criteria
Healthy females; aged 25 - 50 years; willing to refrain from consuming polyphenolic-rich food/beverages for 24 hours prior to each study day(e.g. red and/or white wine); females who drink red and white wine.
Exclusion Criteria
Cigarette smoker currently or within the past six months; receiving medical treatment including anti-folate drugs currently or within the past six months e.g. aminopterin (AMT) and methotrexate (MTX); planning a pregnancy or currently pregnant; females allergic to compounds present in red and white wines.
Study & Design
- Study Type
- Interventional
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method Primary outcome measures will be oxidative dna damage which will be measured by determining micronucleus frequency using the cytokinesis-block micronucleus assay scored by visual microscopy.[Timepoint -10mins - fasting baseline blood sample; Time point 0 - consume 600ml study drink or control within 10 minutes;<br>Timepoint 1.0hr post drink blood sample; <br>Timepoint 2.0hr post drink blood sample.]
- Secondary Outcome Measures
Name Time Method the secondary outcome measures will be nucleoplasmic bridges, nuclear buds, cell death and nuclear division index, which will be measured using the cytokinesis-block micronucleus assay scored by visual microscopy.[Timepoint -10mins - fasting baseline blood sample; Time point 0 - consume 600ml study drink or control within 10 minutes;<br>Timepoint 1.0hr post drink blood sample; <br>Timepoint 2.0hr post drink blood sample.]