Analysis of Proteome and Pathophysiological Characteristics of Human Blood Cells

Completed

• Conditions: Endothelial NO-synthase; Red Blood Cell; Peripheral Blood Mononuclear Cell

• Registration Number: NCT02272530
• Lead Sponsor: Heinrich-Heine University, Duesseldorf

Brief Summary

The aim of this study is to investigate wether change in endothelial nitric oxid synthase of blood cells (erythrocytes and peripheral blood mononuclear cells (PBMC)) has an influence on development and progression of cardiovascular diseases.

Detailed Description

Not available

Study Timeline

• Study Start: 2012-07
• Study First Submitted: 2014-10-21
• Study First Submitted QC: 2014-10-21
• Study First Posted: 2014-10-23
• Primary Completion: 2020-12-31
• Study Completion: 2020-12-31
• Status Verified: 2021-02
• Last Update Submitted: 2021-02-15
• Last Update Posted: 2021-02-16

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 200

Inclusion Criteria

• healthy volunteers
• Patients: stable coronary artery disease with accordingly endothelial dysfunction

Exclusion Criteria

• acute inflammation (CRP>0,5 mg/dl, leucocyte >11000/dl)
• malignant diseases
• acute or terminated kidney disease
• relevant heart failure (NYHA III-IV)

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
Blood examination	baseline	Following parameters will be examined: * Blood count (red cells, white cells, platelets, hemoglobin, hematocrit, mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), red blood cell distribution width (RDW), mean platelet volume (MPV) ); * Creatinin; * Urea; * Glucose; * Glycated hemoglobin (HbA1c); * Cholesterol; * Triglyceride; * C-reactive Protein; * Apolipoprotein

Secondary Outcome Measures

Name	Time	Method
Protein expression	baseline	Determination with western blot
Nitric oxid metabolite and proteins in blood plasma, red blood cells and peripheral blood mononuclear cells (PBMN)	baseline	Determination with reductive chemiluminescence
Asymmetric dimethylarginine (ADMA) and Cytokine	baseline	Determination with enzyme-linked immunosorbent assay (ELISA).
Microparticles	baseline	Determination with ELISA and flow cytometry
Nitric oxid and reactive oxygen species	baseline	Determination with fluorescence coloring in flow cytometry and fluorescence microscopy
Immunocytochemistry	baseline

Trial Locations

Locations (1)

University Hospital Duesseldorf; 🇩🇪 Duesseldorf, Germany