Study the Effect of Oral Zinc Supplementation on High Molecular Weight Zinc Binding Protein in Semen

Completed

• Conditions: Asthenozoospermia
• Interventions: Dietary Supplement: zinc sulfate

• Registration Number: NCT01612403
• Lead Sponsor: mahmoud hussein hadwan

Brief Summary

There are several causes leading to male infertility, like oxidative stress, and nutritional deficiency of trace elements like zinc. Zinc in human seminal plasma was divided into three types of ligands which are high (HMW), intermediate (IMW), and low molecular weight ligands (LMW). The present study was conducted to study the effect of Zn supplementation on the quantitative and qualitative characteristics of semen along with Zinc Binding Protein levels in semen of patients with asthenozoospmia.

Detailed Description

Elevation of ROS levels lead to an increase the oxidation of HMW-Zn binding proteins in seminal plasma of asthenozoospermic subjects. Zinc supplementation restores HMW-Zn% in seminal plasma of asthenozoospermic subjects to normal value. LMW-Zn% is elevated in seminal plasma of asthenozoospermic patients. It may be because increment the levels of semenogelin in seminal plasma of asthenozoospermic subjects. Zinc supplementation elevates LMW-Zn% in seminal plasma of asthenozoospermic subjects to more than normal value, this may be because of its enhancement of the synthesis of metallothioneins (Low molecular weight zinc binding protein).

Study Timeline

• Study Start: 2011-07
• Primary Completion: 2012-04
• Study Completion: 2012-04
• Status Verified: 2012-06
• Study First Submitted: 2012-06-01
• Study First Submitted QC: 2012-06-04
• Study First Posted: 2012-06-05
• Last Update Submitted: 2012-06-05
• Last Update Posted: 2012-06-06

Recruitment & Eligibility

• Status: COMPLETED
• Sex: Male
• Target Recruitment: 37

Inclusion Criteria

The inclusion criteria were the presence of asthenozoospermia in the semen sample.

Exclusion Criteria

• the absence of endocrinopathy,
• varicocele, and
• female factor infertility. Smokers and alcoholic men were excluded from the study because of their recognized high seminal ROS levels and decreased antioxidant levels.

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Arm && Interventions

Group	Intervention	Description
Single group	zinc sulfate	Single group: all participants receive same intervention throughout study (Non-randomised)

Primary Outcome Measures

Name	Time	Method
high molecular weight zinc binding protein	at the end of three months	elevated levels of high molecular weight zinc binding protein to normal value

Secondary Outcome Measures

Name	Time	Method
qualitative semen parameters	at the end of three months	Volume of semen, progressive sperm motility percentage and total normal sperm count increased after zinc sulfate supplementation.

Trial Locations

Locations (2)

Babylon University; 🇮🇶 Hilla, Iraq

Babylon university/ college of science; 🇮🇶 Hilla, Iraq