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Skeletal Muscle Metabolism during Rest, Exercise and Recovery in Patients with Cystic Fibrosis and Healthy Controls

Completed
Conditions
mucoviscidosis
10028302
10010613
Registration Number
NL-OMON36204
Lead Sponsor
niversitair Medisch Centrum Utrecht
Brief Summary

Not available

Detailed Description

Not available

Recruitment & Eligibility

Status
Completed
Sex
Not specified
Target Recruitment
20
Inclusion Criteria

Patients with CF:
- Ambulant patients with CF
- free from acute pulmonary or gastro-intestinal exacerbation
- from 12 to 18 years
Healthy controls:
- from 12 to 18 years
- free from constraints in performing a maximal exercise test

Exclusion Criteria

Patients with CF:
- Resting oxygen saturation (SpO2)< 94%
- FEV1predicted < 80%
- Ineligible to perform an exercise test
- Not familiar with the Dutch language
- Presence of contra-indications for 31P MRS measurements (assessed by a standardised questionnaire as previously used in METC 08-267/K)
- Participation in DO-IT or having participated less than six months ago in DO-IT (METC 09-114/K);Healthy controls:
- Ineligible to perform a maximal exercise test (assessed by a standardised questionnaire as previously used in METC 10-468)
- Not familiar with the Dutch language
- Presence of contra-indications for 31P MRS measurements (assessed by a standardised questionnaire as previously used in METC 08-267/K)

Study & Design

Study Type
Observational non invasive
Study Design
Not specified
Primary Outcome Measures
NameTimeMethod
<p>- * time of PCr recovery (31P MRS)<br /><br>- Resting, exercise and recovery phoscreatine (PCr)(31P MRS)<br /><br>- Resting, exercise and recovery Inorganic phosphorus (Pi)(31P MRS)<br /><br>- Resting, exercise and recovery Pi:PCr ratio(31P MRS)<br /><br>- Resting, exercise and recovery (Adenosine triphosphate) ATP(31P MRS)<br /><br>- Resting, exercise and recovery ATP:PCr ratio(31P MRS)Change in concentration<br /><br>deoxygenated hemoglobin. (NIRS)<br /><br>- Change in concentration oxygenated hemoglobin. (NIRS)<br /><br>- Change in concentration total hemoglobin. (NIRS)<br /><br>- Change in concentration deoxygenated hemoglobin minus change in concentration<br /><br>oxygenated hemoglobin. (NIRS)<br /><br>- The T1/2 (s) change in oxygenated hemoglobin in the recovery phase. (NIRS)</p><br>
Secondary Outcome Measures
NameTimeMethod
<p>- Peak workload (Wpeak)<br /><br>- Resting and peak oxygen uptake (VO2peak/kg (ml.min-1.kg-1) )<br /><br>- Resting and peak minute ventilation (VEpeak) (l.min-1)<br /><br>- Resting and peak respiratory exchange ratio (RER = VCO2/VO2)<br /><br>- Resting and peak heart rate (HR; bpm)<br /><br>- Resting and peak SpO2</p><br>
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