The Effect of Smoking on Gingival Crevicular Fluid Peptidoglycan Recognition Protein-1 Level Following Initial Periodontal Therapy in Chronic Periodontitis
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 发起方
- 入组人数
- 80
- 主要终点
- Change in peptidoglycan recognition protein-1
研究概览
简要总结
A total of 40 patients with CP (20 smokers (S+CP) and 20 nonsmokers (S-CP)) and 40 periodontally healthy subjects (20 smokers (S+PH) and 20 nonsmokers (S-PH)), comprising 80 subjects, were included in this study. Baseline GCF samples were obtained from all subjects and clinical periodontal measurements, including probing depth (PD), clinical attachment level (CAL), plaque index (PI), and gingival index (GI), were recorded. In patients who had received initial periodontal therapy, GCF samples were obtained and all clinical periodontal measurements were recorded again during the 6th-8th weeks. GCF PGRP-1 levels were analyzed by enzyme-linked immunosorbent assay.
详细描述
Peptidoglycan recognition proteins (PGRPs) are one of the antimicrobial peptides that play a role in innate immunity and act against the bacterial cell wall. Smoking is the most important environmental risk factor for periodontitis. This study determined the effect of smoking on PGRP-1 levels in gingival crevicular fluid (GCF) in smokers and nonsmokers with chronic periodontitis (CP) following initial periodontal therapy.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Parallel
- 主要目的
- Treatment
- 盲法
- None
入排标准
- 年龄范围
- 22 Years 至 56 Years（Adult）
- 接受健康志愿者
- 是
入选标准
- •Clinical diagnosis of severe chronic periodontitis
- •Clinical diagnosis of periodontal health
排除标准
- •have any systemic disease,
- •to be alcohol consumer,
- •to receive any periodontal therapy in the past 6 months,
- •to take any drugs regularly
- •to be pregnant and/or lactate.
研究组 & 干预措施
Smokers with chronic periodontitis
Patients who had teeth with 30% periodontal bone loss, presence of at least two nonadjacent sites per quadrant with probing pocket depths of ≥5 mm, and bleeding on probing were assigned as chronic periodontitis group Patients who have smoked for at least 10 years and a minimum of 10 cigarettes per day were assigned as current smokers.
干预措施: Initial periodontal therapy (Other)
Non-smokers with chronic periodontitis
Patients who had teeth with 30% periodontal bone loss, presence of at least two nonadjacent sites per quadrant with probing pocket depths of ≥5 mm, and bleeding on probing were assigned as chronic periodontitis group Never smoked patients were included into the nonsmoker group
干预措施: Initial periodontal therapy (Other)
Smokers with periodontal healthy
Individuals were diagnosed periodontally healthy as without attachment loss, periodontal disease history, whole mouth bleeding scores of <10% and a probing depth of ≤3 mm Patients who have smoked for at least 10 years and a minimum of 10 cigarettes per day were assigned as current smokers.
Non-smokers with periodontal healthy
Individuals were diagnosed periodontally healthy as without attachment loss, periodontal disease history, whole mouth bleeding scores of <10% and a probing depth of ≤3 mm Never smoked patients were included into the nonsmoker group
结局指标
主要结局
Change in peptidoglycan recognition protein-1
时间窗: Baseline and 3 months
Total amount of Peptidoglycan Recognition Protein-1 (µg) in gingival crevicular fluid
次要结局
- Change in periodontal clinical parameter(Baseline and 3 months)
研究者
Meltem Karsiyaka Hendek
Assistant Professor
Kırıkkale University