Apoptosis and Tissue Destruction in Gingival Tissues of Smoker and Non-smoker Periodontitis Patients
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 发起方
- 入组人数
- 60
- 试验地点
- 1
- 主要终点
- Fibroblast and total inflammatory cell counts
研究概览
简要总结
Smoking is a major environmental risk factor associated with common forms of human chronic periodontitis. The aim of the present study was to evaluate apoptotic tissue alterations and tissue destruction in smoker and non-smoker chronic periodontitis patients and healthy individuals. The investigators of the study suggest that smoking decrease tissue quality and increase inflammation level in gingival tissues in both healthy individuals and periodontitis patients. One possible mechanism for this is suggested to be increased apoptosis.
详细描述
Periodontal disease disrupts soft tissue metabolism in the gingiva through a decrease in the production of collagen, the quality, and quantity of the connective tissue. The etiology and pathogenesis of chronic periodontitis are mostly revealed, however, the mechanism of environmental factors such as smoking yet to be clarified. Major consequences of smoking in gingival tissues are suggested to be the reduction in neutrophil and fibroblast function, decreased immunoglobulin G production, increased periodontal pathogen bacteria prevalence, difficulty in eliminating pathogens with mechanical therapy, and reduction in growth factor production. In the present study, markers of tissue destruction, matrix metalloproteinase-8 and tissue inhibitor of matrix metalloproteinase-1, hypoxia markers, vascular endothelial growth factor and hypoxia-inducible factor and apoptotic markers, bax, bcl-2, and caspase-3 were evaluated.
研究设计
- 研究类型
- Observational
- 观察模型
- Case Control
- 时间视角
- Cross Sectional
入排标准
- 年龄范围
- 30 Years 至 50 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Age range from 30 to 45,
- •the existence of at least 20 functioning teeth,
- •systemical health,
- •no antibiotic use within 6 months,
- •no periodontal therapy within 6 months,
- •no pregnancy or lactation,
- •no drug use, in addition; for the smokers existence of the smoking condition for at least five years.
排除标准
- •Patients younger than 30 older than 50 years old,
- •the absence of occlusion,
- •drug use,
- •pregnancy/lactation,
- •previous antibiotic use,
- •previous periodontal therapy,
- •the existence of any systemical disease.
结局指标
主要结局
Fibroblast and total inflammatory cell counts
时间窗: Biopsies were obtained a day after initial examinations, histological analysis were performed 2 weeks after.
Fibroblast and total inflammatory cell counts in the groups were determined in a standardized 1000 micrometer square area with histomorphometric evaluation.
次要结局
- Apoptotic markers(Biopsies were obtained a day after initial examinations, histological analysis were performed 2 weeks after)
- Hypoxia and tissue destruction markers(Biopsies were obtained a day after initial examinations, histological analysis were performed 2 weeks after)
研究者
Hatice Balci Yuce
Principal investigator
Tokat Gaziosmanpasa University