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Short-term metabolism of long-chain polyunsaturated fatty acids and oxylipins

Not Applicable
Completed
Conditions
prevention of civilization diseases
Registration Number
DRKS00012257
Lead Sponsor
Institut für Lebensmittelwissenschaft und Humanernährung
Brief Summary

Not available

Detailed Description

Not available

Recruitment & Eligibility

Status
Complete
Sex
Male
Target Recruitment
13
Inclusion Criteria

Non smoking; omnivores with moderate meat consumption (2x per week) for at least 5 years and low fish consumption (max 1x per week.); written consent of the subjects after detailed oral and written explanation of the course content, requirements and risks; ability and willingness of study participants to follow the instructions of the investigator (compliance with the conditions of the study, taking the test products according to the dosage recommendation, etc.)

Exclusion Criteria

severe chronic diseases (manifest cardiovascular diseases, cancer); insulin-dependent individuals with diabetes mellitus type I and II; serious kidney or liver failure, liver disease, chronic diseases of the gastrointestinal tract (especially the small intestine, liver, pancreas) and conditions after surgery on the gastrointestinal tract; e.g. gastrectomy, celiac disease, enterocolitis, chronic pancreatitis, cholestasis, short bowel syndrome, inflammatory bowel disease; not: appendicitis, gallstone surgery; surgery for stomach stapling, gastric band, gastric balloon; known endocrine and immunological disorders; immediate need of a surgical procedure; simultaneous participation in another clinical trial or participation within the past 30 days; not incapacitated subjects; refusal or withdrawal of consent by the subject; patients taking preparations that contain LC n-3 PUFA, phytosterols, polyglucosamine (chitosan), vitamin E or other lipid-lowering ingredients include; triglyceride (TG) levels = 150 mg / dL (= 1.7 mmol / l); total cholesterol (TC) levels = 200 mg / dL (= 5.2 mmol / l); body mass index (BMI): = 20 kg / m2 and = 27 kg / m2; relative AA content in erythrocyte membranes: 15 ± 3%; Relative EPA / DHA content in erythrocyte membranes (Omega-3 Index): 6 ± 2%; liver enzymes: aspartate aminotransferase (AST) (> 50 U / L), alanine aminotransferase (ALT) (> 50 U / L), ?-glutamyl transferase (GGT) (> 60 U / L); hormonal disorders, such as hyperthyroidism and Cushing's syndrome; regular intake of laxatives; regular intake of nonsteroidal anti-inflammatory drugs (NSAIDs), such as ibuprofen, aspirin, flurbiprofen, diclofenac; chronic administration of corticosteroids (except inhalative), psychotropic drugs; treatment with lipid lowering agents (e.g., statins, fibrates, Gallensäureaustauscherharze, ezetimibe) in the last three months; diagnosed blood clotting disorders; taking anticoagulants (anticoagulant drugs), as phenprocoumon and platelet aggregation inhibitors, for example, ASS; alcohol, drugs and / or drug abuse

Study & Design

Study Type
interventional
Study Design
Not specified
Primary Outcome Measures
NameTimeMethod
EPA and DHA-oxylylins in plasma at baseline and at 7 different time points (2, 4, 6, 8, 24, 48 and 72 hours) after consumption of the test product.<br>EPA-metabolites: Resolvin E1, PGE3, PGD3, 5-HEPE, 8-HEPE, 12-HEPE, 15-HEPE, 8(9) -EpETE, 11(12) -EpETE, 14(15) -EpETE, 17(18) -EpETE, 5,6-diHETE, 5,15-diHETE, 8,9-diHETE, 8,15-diHETE, 11,12-diHETE, 14,15-diHETE, 17,18-diHETE<br>DHA-metabolites: 10(11) -EpDPE, 13(14) -EpDPE, 16(17) -EpDPE, 19(20) -EpDPE, 4,5-DiHDPE, 7,8- DiHDPE, 10,11-DiHDPE, 13,14-diHDPE, 16,17-diHDPE, 19,20-diHDPE
Secondary Outcome Measures
NameTimeMethod
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