Sleep Deprivation's Regulation of Immune System Function and Behavior

Not Applicable

• Conditions: Sleep Deprivation; Sleep
• Interventions: Behavioral: Neuroeconomics task; Behavioral: Portion Size Task; Procedure: Blood sample

• Registration Number: NCT01730742
• Lead Sponsor: Uppsala University

Brief Summary

The study proposes to investigate whether sleep deprivation will affect a variety of measures, including hormones, immune system functioning, and behaviors related to food intake and hunger. It is predicted that sleep deprivation will affect circulating neutrophil activity, and do so via affects on DNA methylation. It is also predicted that sleep deprivation will up-regulate ghrelin, and down-regulate circulating oxytocin. Finally, it is predicted that sleep deprivation will increase participants' tendencies to pick larger portions of food, and also increase their tendency to purchase foods that are more caloric in a mock supermarket scenario.

Detailed Description

Not available

Study Timeline

• Study Start: 2012-02
• Study First Submitted: 2012-10-11
• Status Verified: 2012-11
• Study First Submitted QC: 2012-11-15
• Last Update Submitted: 2012-11-15
• Study First Posted: 2012-11-21
• Last Update Posted: 2012-11-21
• Primary Completion: 2013-03
• Study Completion: 2013-03

Recruitment & Eligibility

• Status: UNKNOWN
• Sex: Male
• Target Recruitment: 18

Inclusion Criteria

• Male
• Age 18-35y
• Healthy (self-reported) and not on medication
• Non-smoking
• Normal sleep-wake rhythm (i.e. 7-8 h per night, self-reported)

Exclusion Criteria

• Major illness
• Taking any serious medications
• Any sleep conditions (e.g. irregular bedtimes, sleep complaints)
• Any dietary issues with the food items provided
• A history of endocrine or psychiatric disorders

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: CROSSOVER

Arm && Interventions

Group	Intervention	Description
Sleep deprivation	Portion Size Task	Total sleep deprivation: participants were required to stay up for the entire night before a 'Blood Sample' was taken and the 'Neuroeconomics task' and 'Portion size task' were performed
Sleep deprivation	Blood sample	Total sleep deprivation: participants were required to stay up for the entire night before a 'Blood Sample' was taken and the 'Neuroeconomics task' and 'Portion size task' were performed
Sleep	Neuroeconomics task	Sleep: participants had an 8-h sleep opportunity before a 'Blood Sample' was taken and the 'Neuroeconomics task' and 'Portion size task' were performed
Sleep	Portion Size Task	Sleep: participants had an 8-h sleep opportunity before a 'Blood Sample' was taken and the 'Neuroeconomics task' and 'Portion size task' were performed
Sleep	Blood sample	Sleep: participants had an 8-h sleep opportunity before a 'Blood Sample' was taken and the 'Neuroeconomics task' and 'Portion size task' were performed
Sleep deprivation	Neuroeconomics task	Total sleep deprivation: participants were required to stay up for the entire night before a 'Blood Sample' was taken and the 'Neuroeconomics task' and 'Portion size task' were performed

Primary Outcome Measures

Name	Time	Method
Neutrophil phagocytotic function production	Change in 4 neutrophil phagocytotic function from baseline to 36hours later (after the nighttime intervention)	This project is designed to test the inflammatory capacity of circulating neutrophils. It also aims to investigate if any changes in neutrophil efficacy are governed by alterations in DNA methylation.; Measured during 36 hours (at 1930 day 1, 0730 and 1930 day 2, 0730 day 3)

Secondary Outcome Measures

Name	Time	Method
Portion Size Task	Change in selected portion size from baseline to 1 hr after consuming a caloric preload	Participants will be evaluated on their tendency to choose larger or smaller portions of a variety of meal items on a computer screen. This will be conducted both following sleep deprivation and sleep, and changes over the hour will be compared between these conditions.
Neuro-economics task	Change in purchasing behaviour 1 hr after consuming a caloric preload	Participants will be evaluated on their purchasing behavior with regards to high-calorie and low-calorie food items in a mock supermarket scenario following sleep and sleep deprivation.
Circulating hormone levels	Change in circulating hormone levels from baseline (ie. 1930 - before sleep intervention) to 12hr later (0730 after the nighttime intervention)	Participants will have their circulating hormone levels taken and analyzed, including ghrelin and oxytocin, to determine if sleep deprivation alters hormone levels related primarily to obesity or weight gain

Trial Locations

Locations (1)

Uppsala University; 🇸🇪 Uppsala, Sweden