Microcirculation, Adiposity, and Traditional and Emerging Cardiovascular Risk Factors in Prepubertal Children
Overview
- Phase
- Not Applicable
- Intervention
- Not specified
- Conditions
- Child, Only
- Sponsor
- Rio de Janeiro State University
- Enrollment
- 98
- Primary Endpoint
- Microvascular reactivity by nail fold videocapillaroscopy
- Status
- Completed
- Last Updated
- 8 years ago
Overview
Brief Summary
This is a cross-sectional study aimed to investigate microvascular reactivity in prepubertal children according to adiposity status.
Detailed Description
The investigators aimed to investigate microvascular reactivity in prepubertal and to compare these data in different groups according to adiposity status. Firstly, The investigators recruited only children from 5 to 10 years at prepubertal status. After excluding those with exclusion criteria and the acceptance (from their parents) to participate in the study, a blood sample was collected and an appointment at BioVasc lab was programmed. At this day, participants were subject to nail fold videocapillaroscopy. A method used to investigate microvascular reactivity by a non-invasive mean. After this, participants children were followed to the outpatient's care unit to continue follow-up.
Investigators
Luiz Guilherme Kraemer de Aguiar
Associate professor
Rio de Janeiro State University
Eligibility Criteria
Inclusion Criteria
- •children aged from 5 to 10 years at prepubertal Tanner´s status
Exclusion Criteria
- •regular use of any medication
- •hypertension
- •heart disease
- •renal and blood diseases .the presence of any acute or chronic inflammatory/infectious diseases. .presence of diabetes mellitus.
Outcomes
Primary Outcomes
Microvascular reactivity by nail fold videocapillaroscopy
Time Frame: 24 months from the beginning of the recruitment period until the ending of the manuscript.
Nailfold videocapillaroscopy is performed using the fourth finger of the left hand in a temperature-controlled environment (22ºC). The following microvascular parameters are determined at resting state: (a) functional capillary density (FCD), the number of capillaries/mm2 with red blood cell flux, evaluated with 250x magnification in a 3-mm area of the distal row of capillaries in three different areas (lateral, central and medial); (b) red blood cell velocity at rest (RBCV) and during post occlusive reactive hyperemia (PORH); c) the peak RBCV after 1-min arterial occlusion (RBCVmax); and (d) the time taken to reach RBCVmax (TRBCVmax). Firstly, a pressure cuff (1-cm wide) is placed around the proximal phalanx and connected to a mercury manometer.
Secondary Outcomes
- Multivariate analysis using Canonical Discriminant analysis(24 months from the beginning of the recruitment period until the ending of the manuscript.)
- Inflammatory and biochemical biomarkers(24 months from the beginning of the recruitment period until the ending of the manuscript.)