Immunometabolism of Exercise and Fitness
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 102
- 试验地点
- 2
- 主要终点
- Metabolic Function
研究概览
简要总结
This study aims to examine the effects of acute aerobic exercise and cardiorespiratory fitness on cellular metabolism of CD8+ T cells and regulatory T cells (Tregs) found in the peripheral blood of humans. In addition, the study will investigate whether the effects of exercise differ based on exercise intensity, as well as whether the effects of exercise and fitness differ between subpopulations of CD8+ T cells and Tregs. Finally, the study aims to examine whether exercise- and fitness-induced changes in cell metabolism relate to changes in cell function.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Crossover
- 主要目的
- Basic Science
- 盲法
- Single (Outcomes Assessor)
入排标准
- 年龄范围
- 20 Years 至 40 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Men and women, aged 20-40 years
- •individuals presenting with average (40th-60th percentile) cardiorespiratory fitness (i.e., VO2max/VO2peak) for their age and sex will be included in acute exercise experiments; individuals with VO2max/VO2peak value >20ml/kg/min will be included in cardiorespiratory fitness experiments
- •individuals categorized as 'low risk' for a cardiovascular event/heart disease when screened by American College of Sports Medicine (ACSM) criteria
排除标准
- •adults under the age of 20 and over the age of 40
- •Individuals with contraindications to exercise testing will be excluded
- •Individuals with musculoskeletal impairments preventing them from exercising on a cycle ergometer
- •Individuals with disease history known to impact the immune system and/or systemic metabolism will be excluded, including autoimmune diseases, HIV, Hepatitis, diabetes, cancer (completed treatment within <6 months prior), hyper- or hypothyroidism
- •Pregnancy
- •Individuals using medications known to impact the cardiopulmonary and/or immune response to exercise with be excluded, including those using medication to control blood pressure (e.g., beta-blockers), daily steroidal and nonsteroidal anti-inflammatories (except daily aspirin), prescription allergy medication, ADHD medication (Ritalin or Adderall), thyroid medication (e.g., Levothyroxine), and chemotherapy (including treatments within the last 6 months).
- •Individuals habitually using tobacco products within the last 6 months or with excessive alcohol intake (>10 drinks/week)
研究组 & 干预措施
Acute effects: Moderate Intensity Exercise
Participants with a moderate fitness level (determined by VO2max value recorded during graded exercise test) will cycle 30minutes exercise below ventilatory threshold. Outcome measures will be compared within-subject across blood sampled before exercise, immediately after exercise, and 2h post exercise. The order of the exercise sessions (above vs below threshold) will be randomized.
干预措施: Acute exercise-moderate intensity (Behavioral)
Acute effects: Heavy intensity exercise
Participants with a moderate fitness level (determined by VO2max value recorded during graded exercise test) will bicycle 30 minutes above ventilatory threshold. Outcome measures will be compared within-subject across blood sampled before exercise, immediately after exercise, and 2h post exercise. The order of the exercise sessions (above vs below threshold) will be randomized.
干预措施: Acute effects-heavy intensity (Behavioral)
结局指标
主要结局
Metabolic Function
时间窗: At rest, immediately after, and two hours after exercise
Cellular metabolic activity and poise of T cells as measured by flow cytometry (SCENITH)
Immune Cell function
时间窗: At rest, immediately after, and two hours after exercise
Stimulated IFN-gamma, perforin and IL-10 production, as measured by flow cytometry
Metabolic Regulation
时间窗: At rest, immediately after, and two hours after exercise
Expression of genes involved in cellular energy metabolism, as assessed by RT-PCR
Mitochondrial Regulation
时间窗: At rest, immediately after, and two hours after exercise
Mitochondrial mass and membrane potential, as assessed by flow cytometry
次要结局
- Peripheral immune cell composition(At rest, immediately after, and two hours after exercise)
研究者
Emily C Lavoy
Associate Professor
University of Houston