Cutibacterium Acnes in Bone and Joint Infections
试验速览
- 阶段
- 不适用
- 发起方
- CHU de Reims
- 入组人数
- 50
- 试验地点
- 1
- 主要终点
- Internalization capacity of C. acnes strains
研究概览
简要总结
Prosthetic Joint Infections (PJIs) are increasing with the use of orthopedic devices on an ageing population. Cutibacterium acnes is a commensal organism that plays an important role in the ecosystem healthy human skin, yet this species is also recognized as a pathogen in foreign body infection: endocarditis, prostatitis and specifically in PJIs. C. acnes is able to escape the immune system. This phenomenon could reflect two bacterial behavior: the bacterial internalization by host cells and the biofilm formation.
详细描述
The aim of the study is to evaluate specific pathogenicity factors (ability to internalize bone cells and ability to form a biofilm) of bacteria derived from clinical strains of C. acnes isolated from bone infections on orthopedic material.
研究设计
- 研究类型
- Observational
- 观察模型
- Case Only
- 时间视角
- Retrospective
入排标准
- 年龄范围
- 18 Years 至 —（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •bone infection with C. acnes,
- •isolation of the strain,
- •presence of a single species,
- •conservation of a viable strain,
- •patients over 18 years
排除标准
- 未提供
结局指标
主要结局
Internalization capacity of C. acnes strains
时间窗: Day 0
C. acnes strains will be cultured for 5 days in rich medium at 37°C in anaerobic conditions. Then, the bacteria will be co cultured with bone cells (osteoblast) and bacteria will be internalized by the bone cells. After killing bacteria stayed outside of the cells with an antibiotic and lysis of the cells, the number of bacteria internalized will be counted on agar plate.
Biofilm capacity of C. acnes strains
时间窗: Day 0
C. acnes strains will be cultured for 5 days in rich medium at 37°C in anaerobic conditions. Then, the biofilm biomass will be evaluated by Crystal violet staining. The amount of biofilm will be evaluated by absorbance (optic density by spectrophotometry).
次要结局
未报告次要终点