Differences in Brain Derived Neurotrophic Factor Levels Between Chronic and Asymptomatic Pain Patients
试验速览
- 阶段
- 不适用
- 状态
- 招募中
- 入组人数
- 66
- 试验地点
- 1
- 主要终点
- BDNF Levels
研究概览
简要总结
A case-control study will be conducted to analyze the differences in BDNF levels between patients with chronic pain and asymptomatic people.
详细描述
Brain-derived neurotrophic factor (BDNF) is considered a biomarker of brain plasticity. Since different alterations in brain plasticity have been found in chronic pain patients, the investigators want to study whether there are differences at the molecular level in this population compared to an asymptomatic population in order to be able to accurately measure the impact of different physiotherapy interventions in an objective way.
研究设计
- 研究类型
- Observational
- 观察模型
- Case Control
- 时间视角
- Cross Sectional
入排标准
- 年龄范围
- 18 Years 至 65 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •males and females aged 18 to 65 years with musculoskeletal pain for a minimum of 3 months
- •patients recruited by information pamphlets from the university clinic of the Rey Juan Carlos University, and CSEU La Salle
- •not having received physiotherapy treatment for this same process in the last 3 months.
- •ability to perform all the clinical tests and to understand the study process, as well as to obtain informed consent.
排除标准
- •Systemic, neurological, oncological or inflammatory diseases; psychiatric pathologies, pregnancy, type II diabetes.
结局指标
主要结局
BDNF Levels
时间窗: 3 hours
Brain Derived Neurotrophic Factor: Pierce NeutrAvidin-coated high-capacity plates (ThermoFisher Scientific; 15509) will incubate for two hours at RT with 200 µl of 14 µg/ml biotin-conjugated mAb-#1 diluted in phosphate buffer (0.1% Triton X-100 in 0.1 M phosphate buffer: 0.1 M KH2PO4 and 0.1 M Na2HPO4; pH 7.6). Plates will then washed three times with blocking buffer \[1% bovine serum albumin (BSA); Sigma A2153 in phosphate buffer\], followed by the addition of 150 µl phosphate buffer. A total of 50 µl of either standards or diluted samples (both in blocking buffer) will then added to the plate followed by incubation for 3 h at RT on a rotary shaker.
Pain Intensity
时间窗: 3 hours
Pain intensity assessed with the 100 mm Visual Analog Scale, in which the patient is asked about his or her pain level at that moment, with one side being "no pain" and the other extreme being "the worst pain imaginable"
次要结局
未报告次要终点
研究者
Raúl Ferrer-Peña
Principal Investigator
Universidad Autonoma de Madrid