Effects of glycogen availability on human skeletal muscle molecular responses to exercise

Not Applicable

Recruiting

• Conditions: Metabolic disorders; Obesity; Metabolic and Endocrine - Metabolic disorders; Musculoskeletal - Normal musculoskeletal and cartilage development and function; Diet and Nutrition - Obesity

• Registration Number: ACTRN12621000432819
• Lead Sponsor: Dr Nolan Hoffman

Brief Summary

Not available

Detailed Description

Not available

Study Timeline

• Results First Posted: 1900-01-01
• Study Start: 2021-04-16
• Last Update Posted: 11 March 2024

Recruitment & Eligibility

• Status: Recruiting
• Sex: Male
• Target Recruitment: 10

Inclusion Criteria

Participants will be recruited if they meet the following criteria:
•Male
•Aged 18-30 y
•BMI: 18.5-27 kg/m2
•Recreationally active (i.e. target V02 max approximately between 40-45 ml/kg)
•No cardiopulmonary abnormalities
•No injuries
•Pass the ESSA pre-exercise screening tool and/or obtain GP clearance to exercise
•Able to ride a stationary cycle ergometer at high intensity and successfully complete the entire duration of the glycogen depletion exercise protocol

Exclusion Criteria

•Known cardiovascular disease or diabetes mellitus
•Known bleeding disorder (i.e. hemophilia A [factor VIII deficiency]
•Hemophilia B [factor IX deficiency]
•von Willebrand disease, or other rare factor deficiencies including I, II, V, VII, X, XI, XII and XIII)
•Major or chronic illness that impairs mobility or eating/digestion
•Taking prescription medications (i.e. beta-blockers, anti-arrhythmic drugs, statins, insulin sensitising drugs, or drugs that increase the risk of bleeding [i.e. anticoagulants, antiplatelets, novel oral anticoagulants [NOAs], nonsteroidal anti-inflammatory drugs [NSAIDs], selective norepinephrine reuptake inhibitors [SNRI], or selective serotonin reuptake inhibitors [SSRIs]
•Unable or unwilling to have a muscle biopsy
•Weight has changed more than 5 kg in past 3 months
•Currently on a restrictive diet
•Unable to attend the lab based on ACU's campus in Fitzroy (inner suburb of Melbourne), Victoria for the 5 study visits for completion of study protocol

Study & Design

• Study Type: Interventional
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
Determine the effects of a single session of exercise on protein post-translational modifications (i.e. protein phosphorylation) in skeletal muscle with low versus high glycogen availability.[ Skeletal muscle biopsy samples will be obtained before and immediately following exercise from both the low and high glycogen legs to measure protein post-translational modifications (i.e. phosphorylation).];Determine the effects of a single session of exercise on protein content in skeletal muscle with low versus high glycogen availability.[ Skeletal muscle biopsy samples will be obtained before and immediately following exercise from both the low and high glycogen legs to measure protein content.]

Secondary Outcome Measures

Name	Time	Method
Determine the effects of a single session of exercise on gene expression (i.e. transcription) in skeletal muscle with low versus high glycogen availability.[ Skeletal muscle biopsy samples will be obtained before, immediately following and 3 h post-exercise from both the low and high glycogen legs to measure gene expression (i.e. transcription).];Determine the effects of a exercise on plasma hormone and metabolite concentrations. This is a composite and exploratory secondary outcome.[ Blood samples will be obtained before, immediately following and 3 hours following two-legged exercise to measure the effects of exercise on plasma hormones and metabolites. ]