Human Brown Adipose Tissue and Mitochondrial Respiration

• Conditions: Mitochondrial Metabolism Disorders; Obesity; Adipose Tissue, Brown; Aging

• Registration Number: NCT03111719
• Lead Sponsor: Maastricht University Medical Center

Brief Summary

This study is focused on measuring mitochondrial activity of human brown adipose tissue. During surgery, adipose tissue biopsies from white and brown adipose tissue will be collected from the deep neck area. These biopsies will be used to examine mitochondrial function via histological analysis, biochemical analysis and in vitro experiments based on precursor cells present in the biopsies.

Detailed Description

Not available

Study Timeline

• Study Start: 2010-01-04
• Study First Submitted: 2017-03-30
• Study First Submitted QC: 2017-04-07
• Study First Posted: 2017-04-13
• Status Verified: 2020-02
• Last Update Submitted: 2020-02-20
• Last Update Posted: 2020-02-21
• Primary Completion: 2021-10-10
• Study Completion: 2021-10-10

Recruitment & Eligibility

• Status: UNKNOWN
• Sex: All
• Target Recruitment: 180

Inclusion Criteria

• Indication for surgery in the supraclavicular and neck region (more specific patients diagnosed with hyperparathyroidism, struma, benign thyroid gland tumours, implantation of a vagal neurostimulator for epilepsia and cervical neurologic disorders)
• In the thyroid gland group, euthyroid function

Exclusion Criteria

• Pre-operatively diagnosed malign tumour
• Patients undergoing acute surgery because of critical illness

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
Mitochondrial respiration in human brown	8 weeks	Measure of mitochondrial respiration in human brown adipose tissue biopsies and measure mitochondrial respiration in cells derived from human brown adipose tissue biopsies. With high resolution respirometry , oxygen consumption in mitochondria will be determined using different substrates that stimulate mitochondrial activity including norepinephrine which is a natural agonist to increase mitochondrial activity in brown adipose tissue. During these experiments mitochondrial complex activity can be inhibited using specific inhibitors.

Secondary Outcome Measures

Name	Time	Method
Characterise human brown adipose tissue	8 weeks	Perform histological analysis and biochemical analysis in brown adipose tissue biopsies and compare to skeletal muscle. Via qPCR analysis, western blotting and immunfluorescence we will examine UCP1 expression in BAT.

Trial Locations

Locations (1)

Maastricht University Medical Center / University of Maastricht; 🇳🇱 Maastricht, Netherlands