Mitochondrial Oxygraphy on Subcutaneous and Peri-visceral Adipose Tissue: Influence of Body Composition

Not Applicable

Terminated

• Conditions: Bariatric Surgery Candidate; Digestive Organs--Cancer; Parietal Region Trauma
• Interventions: Procedure: adipose surgery

• Registration Number: NCT05417581
• Lead Sponsor: University Hospital, Clermont-Ferrand

Brief Summary

The objective of this study is to adapt the oxygraphy technique on human adipocytes and to characterize respiration measurements according to patients' body mass index and white adipose tissue localization (subcutaneous vs visceral).

Detailed Description

During digestive surgery: oncological and bariatric surgery, it is possible to collect a small volume of subcutaneous and epiplonic adipose tissue (10-20 cc). We wish to collect these samples in order to develop the technique of oxygenography on permeabilized adipocytes. In order to characterize these measurements, they will be related to the clinical data of the patients usually collected in the framework of the preoperative assessment including the body mass index (BMI). The measured adipocyte data will thus be normalized to three BMI classes: ≤ 20, \> to 20 \< 30, and ≥ to 30 kg∙m-2.

Primary end point is to validate the measurement of mitochondrial respiration under oxidophosphorylating conditions by determining indicators of the distribution of mitochondrial respiration values (mean, standard deviation, skewness, kurtosis, coefficient of variation) as a function of patients' body mass index.

Study Timeline

• Study Start: 2021-04-14
• Study First Submitted: 2022-05-17
• Study First Submitted QC: 2022-06-09
• Study First Posted: 2022-06-14
• Primary Completion: 2023-04-12
• Study Completion: 2023-04-12
• Status Verified: 2023-09
• Last Update Submitted: 2023-09-29
• Last Update Posted: 2023-10-02

Recruitment & Eligibility

• Status: TERMINATED
• Sex: All
• Target Recruitment: 67

Inclusion Criteria

• Patients undergoing oncological, bariatric, parietal surgery
• Patients able to give an informed consent
• Patients affiliated to an Health Care insurance
• Patients aged over 18

Exclusion Criteria

• Patients under guardianship, curatorship or deprived of freedom
• Pregnant or breastfeeding women
• Participation refusal
• Patients under chemotherapy
• Patients with peritonitis and infectious parietal processes

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: SINGLE_GROUP

Arm && Interventions

Group	Intervention	Description
All patients	adipose surgery	a sample of adipose tissue will be collected during the scheduled surgery

Primary Outcome Measures

Name	Time	Method
Evaluation of mitochondrial respiration on adipose tissue by oxygraphy	day 1	Mitochondrial respiration measurements in oxydophosphorylated conditions. For each patient measurements using ADP and carbohydrate or lipid substrates of the respiratory chain complexes for both the subcutaneous and the visceral tissue.

Secondary Outcome Measures

Name	Time	Method
mitochondrial respiration values depending on patients BMI by oxygraphy	day 1	Identification of mitochondrial respiration discrimination factors
comparing mitochondrial respiration values depending on tissue location by oxygraphy	day 1	Identification of mitochondrial respiration discrimination factors
comparing mitochondrial respiration values depending on anthropometric data by oxygraphy	day 1	Identification of mitochondrial respiration discrimination factors

Trial Locations

Locations (1)

CHU clermont-ferrand; 🇫🇷 Clermont-Ferrand, France