Cutaneous oxygenation and mitochondrial respirometry measurements, using the Protoporphyrin IX - Triplet State Lifetime Technique

Recruiting

• Conditions: gezonde vrijwilligers; Mitochondrial oxygen measurements in the skin; standard values

• Registration Number: NL-OMON37834
• Lead Sponsor: Erasmus MC, Universitair Medisch Centrum Rotterdam

Brief Summary

Not available

Detailed Description

Not available

Study Timeline

• Last Update Posted: 29 April 2024

Recruitment & Eligibility

• Status: Recruiting
• Sex: Not specified
• Target Recruitment: 30

Inclusion Criteria

- Speaking Dutch
- Age between 18 and 30 years.
- Healthy subjects

Exclusion Criteria

- Non healthy subjects

Study & Design

• Study Type: Observational invasive
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
<p>The mean and standard deviation of mitoPO2 will be calculated in mmHg. The<br /><br>mitoVO2 and mitoP50 will be determined by a decrease in mitoPO2 after oxygen<br /><br>supply is blocked to the cells . The mitoPO2 will be recorded before during,<br /><br>and after a 90 second compression. The mitoVO2 is calculated from the decay of<br /><br>the mitoPO2 slope. With the Michaelis-Menten equation we can calculate MitoP50<br /><br>in mmHg.</p><br>

Secondary Outcome Measures

Name	Time	Method
<p>The practical applicability of the method will be tested by the researcher. If<br /><br>necessary adjustments will be made. The raw data of the measurements will be<br /><br>stored for extended off-line analysis of the signal quality. If necessary, we<br /><br>will look at opportunities to optimize the equipment.<br /><br><br /><br>The structure of the fluorescent signal, determines by means of the intensity<br /><br>of the detected signal (V) divided by time (minutes), expressed in V / minute.<br /><br><br /><br>The influence of skin temperature to the search parameters (mitoPO2, and<br /><br>mitoVO2 mitoP50), will be determined at two different skin temperatures (34 and<br /><br>40 degrees celsius ).</p><br>